Contact Inhibition in Microcarrier Cultures of MRC-5 Cells

3 ORDINARY DIFFERENTIAL EQUATION MODELS 17.3.1 Contact Inhibition in Microcarrier Cultures of MRC-5 Cells 

Contact inhibition is a characteristic of the growth of anchorage dependent cells grown on microcarriers as a monolayer. Hawboldt et al. (1994) reported data on MRC5 cells grown on Cytodex II microcarriers and they are reproduced here in Table 17.13. 

Growth inhibition on microcarriers cultures can be best quantified by cellular automata (Hawboldt et al., 1994 Zygourakis et al., 1991) but simpler models have also been proposed. For example, Frame and Hu (1988) proposed the following model 

Time Cell Density Time Cell Density 

Finally, we consider the simulatneous regression of both data sets. Using the converged parameter values from the first data set as an initial guess convergence was obtained in 11 iterations. In this case parameters C and pmax were common to both data sets however, two different values for x were used - one for each data 

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