Mouse keratinocytes

Gupta, A., Rosenberger, S. F., and Bowden, G. T., Increased ROS levels contribute to elevated transcription factor and MAP kinase activities in malignantly progressed mouse keratinocyte cell lines, Carcinogenesis, 20, 2063, 1999. 

Beehler, B.C., Przybyszewski, J., Box, H.B. Kulesz-Martin, F. (1992) Formation of 8-hydroxy-deoxyguanosine within DNA of mouse keratinocytes exposed in culture to UVB and H2O2. Carcinogenesis, 13, 2003-2007... 

DIA, DNA damage (comet assay), male CBA mouse keratinocytes + NT 100 YendleeZa/. (1997)... 

The requirements for epithelial cells are somewhat different (Reiss and Dibble, 1988). Mouse keratinocytes (MK-1 cells) enter a GO-phase within 24 h when confluent cultures are fed a serum-free, low Ca2+ (< 0.1 mM) medium supplemented with insulin, transferrin and sodium selenate (see 5.8). Addition of EGF (10 ng/ml) causes cells to enter S-phase after 10-12 h although the percentage of cells responding is not known. Insulin is not essential for this effect but apparently leads to a threefold increase on the rate of DNA synthesis measured 22-24 h after addition of EGF. TGF/ (100 pM) completely abolishes the effect of EGF. 

Cai, Y.N., Baerdubowska, W., Ashwoodsmith, M. and Digovanni, J. (1997) Inhibitory effects of naturally occurring coumarins on the metabolic activation of benzo[A]pyrene and 7,12-dimethylbenz[A]anthracene in cultured mouse keratinocytes. Carcinogenesis. 18, 215. 

Li M, Messaddeq N, Teletin M, Pasquali JL, Metzger D, Chambon P Retinoid X receptor ablation in adult mouse keratinocytes generates an atopic dermatitis triggered by thymic stromal lymphopoietic Proc Natl Acad Sci USA 2005 102 14795-14800. 

Figure 8. Fluorescence images (CLSM) of primary mouse keratinocytes after 4 h incubation with 0.1 mM ALA. On the left, proliferating keratinocytes show red fluorescence of the cell membranes. Perinuclear yellow autofluorescence indicates lysosomes, and green autofluorescence indicates mitochondria. The right-hand panel shows fluorescence of large, flat, differentiated keratinocytes with accentuated PpIX fluorescence in the cellular and...

B. Ortel, N. Chen, J. Brissette, G.P. Dotto, E. Maytin, T. Hasan (1998). Differentiation-specific increase in ALA-induced protoporphyrin IX accumulation in primary mouse keratinocytes. Br. J. Cancer, 77, 1744-1751. 

Warmka, J.K., et al.. Extracellular signal-regulated kinase transmits palytoxin-stimulated signals leading to altered gene expression on mouse keratinocytes, Toxicol Appl Pharmacol, 185, 8, 2002. 

Zeliadt, N.A., Wanrika, J.K., and Wattenberg, E.V., Mitogen activated protein kinases selectively regulate palytoxin-stimulated gene expression in mouse keratinocytes, Toxicol. Appl. Pharmacol., 212, 192, 2003. 

Dong,Z. etal., A dominant negative mutant of jun blocking 12-0-tetradecanoylphorbol-13-acetate-induced invasion in mouse keratinocytes, ATo/. Carcinog., 19(3), 204,1997. 

Poirier, M. C. Stanley, J. R. Beckwith, J. B. Weinstein, I. B. Yuspa, S. H. Indirect immuno fluorescent localization of benzo-a-pyrene adducted to nucleic-acids in cultured mouse keratinocyte nuclei. Carcinogenesis (Lond), 3 345-8. 1982. 

Mouse keratinocytes 308 (benign) and PAM212 (carcinogenic) Mouse keratinocytes (wild type) More cell cycle arrest In more tumorigenic cells Plasma Jet (He, 13 kHz) [61]... 

Black, A.T., Joseph, L.B., Casillas, R.P., et al., 2010b. Role of MAP kinases in regulating expression of antioxidants and inflammatory mediators in mouse keratinocytes following exposure to the half mustard, 2-chloroethyl ethyl sulfide. Toxicol. Appl. Pharmacol. 245, 352-360. 

Shah PK, Borchardt RT. A comparison of peptidase activities and peptide metabolism in cultured mouse keratinocytes and neonatal mouse epidermis. P harm Res. 1991 8(l) 70-5. 

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