Fluorescence scans

Fluorescence scanning of chromatograms of polycyclic aromatic compounds is a vivid example of their employment. A careful choice of the wavelengths of exci-... 

Fig. 29 Fluorescence scans of polycyclic aromatic hydrocarbons at various excitation wavelengths in combination with various secondary filters.

Fig. 35 Fluorescence scan of the dansyl semicadavende denvatives of 1 behenic acid 2 erucic acid, 3 steanc acid, 4 oleic acid, 5 linoleic acid, 6 linolenic acid...

Fig. 53 Fluorescence scan of femtogram quantities of 2,1,3-naphthoselenodiazole (A) and associated calibration curve (B).

Fig. 1 Separation of flavonoids fluorescence scanning curve of rutin (1), hyperoside (2), quercitrin (3) and quercetin (4).

Fig. 1 Schematic diagram of the chromatographic separation (A) and the fluorescence scan (B) of a sugar mixture containing 1 pg substance per chromatogram zone. Lactose (1), fructose (2), arabinose (3), xylose (4), rhamnose (5), mixture (G).

Fig. 1 Fluorescence scan of a mixture of alkaloids with ca. 50 ng substance per chromatogram zone morphine (1), 6-monoacetylmorphine (2) and heroin (3).

Fig. 1 Fluorescence scan of a chromatogram track with 50 ng vitamin D3 per chromatogram zone by-products (1,2), vitamin D3 (3).

Fig. 2 Fluorescence scan of a chromatogram of peppermint oil menthol (1), cineole (2), menthone (3), menthyl acetate (4), menthofuran/terpenes (5).

Fig. 1 Fluorescence scan of 30 ng thiamine after vapor-phase derivatization (A) and an accompanying blank track (B).

Fig. 1 Reflectance scan (A) and fluorescence scan (B) of a chromatogram track with 50 ng of each amino acid per chromatogram zone hydroxyproline (1), proline (2).

Fig. 1 Fluorescence scan of a blank track (A) and of a chromatogram track (B) with 500 ng netilmicin and 1 gg gentamycin C complex as sample. Netilmicin (1), gentamycin Ci (2), gentamycin Ci, (3), gentamycin C2 (4), gentamycin (5), impurity in netilmicin (6).

Fig. 1 Fluorescence scan of a mixture of digitalis glycosides. Digoxin (DG3), digoxigenin bis-digitoxoside (DGj), digoxigenin mono-digitoxoside (DGi), digoxigenin (DG).

Fig. 2 Fluorescence scan of a zl -3-ketosteroid mixture after INH treatment. Aldosterone...

Fig. 1 Fluorescence scan of chromatogram tracks with 50, 100 and 200 ng arbutin per chromatogram zone.

Fig. 1 Fluorescence scans of a blank (A) and of a mixture (B) of cholesterol (2), coprostanol (3), 4-cholesten-3-one (4), 5a-cholestan-3-one (5) and coprostanone (6), start (1), solvent front (7).

Rg. 1 Fluorescence scan of (A) a blank and (B) a carbamazepine standard with 200 ng per chromatogram zone. Start (1), carbamazepine (2), solvent front (3). 

Fig. 1 Fluorescence scans of chromatogram tracks with 200 ng ascorbic acid (A) and 200 ng dehydroascorbic acid (B) Start (1), ascorbic acid (2), dehydroascorbic acid (3), -front (4).

Fig.l Fluorescence scan of a chromatogram track with 1 gg substance per chromatogram zone. Start (1) atenolol (2), bunitrolol (3), alprenolol (4) and dipping front (5). 

After the hybridization process, intensity signals from the hybridized RNA/cDNA samples are usually detected by phospho-imaging or fluorescence scanning and independent images are generated. 

Now chelidonine produced an intense green fluorescent chromatogram zone in addition there were other intensely fluorescent zones in the track of the celandine extracts — some of which were not previously visible or had another color shade (Fig. IC). In addition the general fluorescence was increased as a result of the UV irradiation. Figure II illustrates the corresponding fluorescence scans. 

Fig. I Fluorescence scan of a blank track (A) and of a chromatogram track with 4 ng testosterone.

Fig. II Fluorescence scan of a chromatogram track with SOO ng each of noradrenaline (1), adrenaline (2), serotonin (3), vanilmandelic add (4), 5-hydroxyindoleacetic add (6), homovanillic acid (7) and vanillic acid (8) together with 230 ng creatinine, all per chromatogram zone measurement at X(3,c = 313 nm and > 390 nm (cut off filter FI 39 (A)), X = 365 nm and Xj, >430 nm...

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