Moloney murine leukaemia virus

Other marine organisms and microorganisms besides algae have also been found to be active against several viruses, such as marine Vibrio spp. which was active against infections caused by the hematopoietic necrosis virus and Oncorhynchus masou virus [129], and the aqueous extracts of marine cephalopods, which inhibited the Moloney murine leukaemia virus RT activity [130]. 

Toh, H., Kikuno, R., Hayashida, H., Miyata, T., Kugimiya, W., et al (1985) Close structural resemblance between putative polymerase ol a Drosophila transposable genetic element 17.6 and pol gene produci of Moloney murine leukaemia virus. EMBO J. 4 1267-1272. 

A further important step in quantitative RT-PCR is the production of a single-stranded (ss) complementary DNA copy (cDNA) of the RNA through the reverse transcriptase (RT). Its dynamic range, sensitivity, and specificity are of prime consideration for a successful kinetic RT-PCR assay. For many quantitative applications, moloney murine leukaemia virus (MMLV) RT is the enzyme of choice, as its cDNA synthesis rate is up to 50-fold greater than that of avian myeloblastosis virus (AMV). Newly available thermostable enzymes maintain their activity up to 70°C, thus permitting increased specificity and efficiency of first primer annealing. Fiowever, this enzyme type may be less robust than more conventional ones as it appears to be more sensitive to inhibitors present in RNA preparation. 

Superscript II reverse transcriptase (Life Technologies, Paisley, UK) This enzyme is a recombinant RNase H" reverse transcriptase from Moloney Murine Leukaemia Virus (MMLV) and is supplied with tubes of 0.1 M dithiothreitol (DTT) and 5X reverse transcriptase reaction buffer (250 mM Tris-HCl, pH 8.3, 375 mM KCl and 15 mM MgClj)... 

Hansma et aL [139] described the first use of AFM to characterise two forms of RNA self-assembly, namely dimerisation of two RNA molecules by the formation of a kissing loop and the formation of a supramoleciflar fibre from tectoRNA. The kissing loop RNA was prepared from a 230 nucleotide Moloney murine leukaemia virus (MoMLV), using polymer chain reactions to generate the desired sequence. 

HIV-1 human immunodeficiency virus-1, SIV simian immunodeficiency virus, MLV murine leukaemia virus, MMLV Moloney MLV, MPMV Mason-Pfizer monkey virus. 

A purified glycoprotein (gp 70) from mouse ascites fluid appears to be related to the principal glycoprotein found in murine leukaemia viruses (Scripps, Moloney, and Rauscher). ... 

Numerous structural modification studies of ellipticine have been conducted. It is found that side-chain substitution has considerable influence on the biological activity to compounds of this type. A pyridopyrrolo[2,3-g]iso-quinoline derivative, BD-40 (48) [235, 236], has demonstrated potent activity against many different experimental tumours including leukaemia L1210 and the Friend virus leukaemia. It is also active against the Moloney strain of murine sarcoma virus [237]. Phase I clinical study of (48) has been conducted [238, 239]. 

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