Proteins quantitative analysis

Peptide Models of bZIP Proteins Quantitative Analysis of DNA Affinity and Specificity... 

Manning JM et aJ Normal and abnormal protein subunit interactions in hemoglobins.] Biol Chem 1998 273 19359-Mario N, Baudin B, Giboudeau J Qualitative and quantitative analysis of hemoglobin variants by capillary isoelectric focusing. J Chromatogr B Biomed Sci Appl 1998 706 123-Reed W, Vichinsky EP New considerations in the treatment of sickle cell disease. Annu Rev Med 1998 49 46l. 

An important technique for the qualitative and quantitative analysis of different macromolecular materiafs is based on the efectrophoretic separation of particfes having different transport vefocities (e.g., because they have different zeta potentiafs). This technique is used for the anafysis of proteins, pofysaccharides, and other naturally occurring substances whose molecular size approaches that of colloidal particles (for more details, see Section 30.3.4). It is an advantage of the electrophoretic method that mild experimental conditions can be used—dilute solutions with pH values around 7, room temperature, and so on—which are not destructive to the biological macromolecules. 

Biinger, H., Kaufner, L., and Pison, U., Quantitative analysis of hydrophobic pulmonary surfactant proteins by high-performance liquid chromatography with light-scattering detection, /. Chromatogr. A, 870, 363, 2000. 

Gygi, S. P., Rist, B., Gerber, S. A., Turecek, F., Gelb, M. H., and Aebersold, R. (1999). Quantitative analysis of complex protein mixtures using isotope-coded affinity tags. Nat. Biotechnol. 17, 994-999. 

Goodacre, R. Karim, A. Kaderbhai, M. A. Kell, D. B. Rapid and quantitative analysis of recombinant protein expression using pyrolysis mass spectrometry and artificial neural networks Application to mammalian cytochrome b5 in Escherichia coli. J. Biotechnol. 1994,34,185-193. 

A detailed quantitative analysis of the preferences of amino acids in folded proteins for different regions of the Ramachandran plot reveals that the 18 nonglycine, nonproline residues exhibit different preferences (Shortle, 2002). Figure 5 shows the range of relative propensities displayed by these 18 amino acids for a somewhat arbitrary subdivision... 

Quantitative analysis of protein IR and VCD spectra in terms of the fractional components (FC) of their secondary structure has taken different approaches, as noted earlier. The FTIR approach of assigning frequencies to specific components can, in principle, identify amounts of unordered structure in a protein fold. The viability of this approach... 

Wang, H., Clouthier, S.G., Galchev, V., Misek, D.E., Duffner, U., Min, C.K., Zhao, R., Tra, J., Omenn, G.S., Ferrara, J.L., Hanash, S.M. (2005). Intact-protein-based high-resolution three-dimensional quantitative analysis system for proteome profiling of biological fluids. Mol. Cell. Proteomics 4, 618-625. 

Shi X, Basran J, Seward FIE, Childs W, Bagshaw CR, Boxer SG (2007) Anomalous negative fluorescence anisotropy in yellow fluorescent protein (YFP 10C) quantitative analysis of FRET in YFP dimers. Biochemistry 46 14403-14417... 

TfR is low in these patients, PIT is normal and most iron is stored in hepatocytes. In patients with hypoplastic anaemias and with transfusion iron overload, the BM cannot utilize iron, resulting in low TfR expression and decreased iron absorption. Quantitative analysis of all iron fluxes, which can be deduced from Figure 9.1, can assist in understanding the clinical expression of mutations of proteins involved in iron transport. 

Leung, A. K., Calabrese, J. M., and Sharp, P. A. (2006). Quantitative analysis of Argonaute protein reveals microRNA-dependent localization to stress granules. Proc. Natl. Acad. Sci. L7 4 103, 18125-18130. 

Cregger M, Berger AJ, Rimm DL. Immunohistochemistry and quantitative analysis of protein expression. Arch. Pathol. Lab. Med. 2006 130 1026-1030. 

Guerrero C, Tagwerker C, Kaiser P, et al. An integrated mass spectrometry-based proteomic approach quantitative analysis of tandem affinity-purified in vivo cross-linked protein complexes (QTAX) to decipher the 26 S proteasome-interacting network. Mol. Cell. Proteomics. 2006 5 366-378. 

For quantitative analysis of protein concentration the colorimetric Bradford-assay [147] is most commonly used. Here another Coomassie dye, Brilliant Blue G-250, binds in acidic solutions to basic and aromatic side chains of proteins. Binding is detected via a shift in the absorption maximum of the dye from 465 nm to 595 nm. Mostly calibration is performed with standard proteins like bovine serum albumin (BSA). Due to the varying contents of basic and aromatic side chains in proteins, systematic errors in the quantification of proteins may occur. 

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