Microscopy techniques comparison

Comparison between Optical Microscopy Techniques for Red Blood Cell Imaging... 

Rowe RC, McMahon J. The characterisation of the microstructure of gels and emulsions containing cetostearyl alcohol and cetrimide using electron microscopy—a comparison of techniques. Colloid Surf 1987 27 367-373. 

In comparison with other microscopy techniques such as optical microscopy or scanning electron microscopy, SPM offers a number of advantages in terms of resolution and... 

In comparison with other microscopy techniques such as optical microscopy or scanning electron microscopy, SPM offers a number of advantages in terms of resolution and magnification. However, due to image acquisition by raster scanning, the measurement is slower and the maximum image size is generally smaller as well. Table 1 list some of the more common applications for SPMs. 

Microscopy is the study of the fine structure and morphology of objects with the use of a microscope. Microscopes range from optical microscopes, which resolve details on the micrometer level, to transmission electron microscopes that can resolve details less than one nanometer across. The size and visibility of the polymer structure to be characterized generally determines which instrument is to be used. For example, the size and distribution of spherulites can be observed by optical techniques, but a study of their internal structure requires electron microscopy. Combinations of the various microscopy techniques generally provide the best insight into the morphology of polymer materials [1]. Table 2.1 shows the basic properties of the different microscopes, for the purpose of comparison. 

Once the objective of the experiment is known and the specimens selected for study, the next major step is the selection of the microscopy techniques and the specimen preparation methods required to image the polymer structures of interest (Table 6.2). If lamellar crystals must be evaluated, for instance, there is no point in considering most optical techniques as they will only provide an overview of these structures. Comparisons are made in this section regarding the various techniques, in both the text and tables, as an aid in this selection process. Observations of... 

Raspanti M, Binaghi E, Gallo 1, Manelli A. (2005) A vision-based, 3D reconstruction technique for scanning electron microscopy Direct comparison with atomic force microscopy. Microsc Res Tech 67 1-7. 

Comparison of FLIM with Other FRET Microscopy Techniques. 165... 

Microscopy (qv) plays a key role in examining trace evidence owing to the small size of the evidence and a desire to use nondestmctive testing (qv) techniques whenever possible. Polarizing light microscopy (43,44) is a method of choice for crystalline materials. Microscopy and microchemical analysis techniques (45,46) work well on small samples, are relatively nondestmctive, and are fast. Evidence such as sod, minerals, synthetic fibers, explosive debris, foodstuff, cosmetics (qv), and the like, lend themselves to this technique as do comparison microscopy, refractive index, and density comparisons with known specimens. Other microscopic procedures involving infrared, visible, and ultraviolet spectroscopy (qv) also are used to examine many types of trace evidence. 

Microscopy is an unusual scientific discipline, involving as it does a wide variety of microscopes and techniques. All have in common the abiUty to image and enlarge tiny objects to macroscopic size for study, comparison, evaluation, and identification. Few industries or research laboratories can afford to ignore microscopy, although each may use only a small fraction of the various types. Microscopy review articles appear every two years m. Jinalytical Chemistty (1,2). Whereas the style of the Enclyclopedia employs lower case abbreviations for analytical techniques and instmments, eg, sem for scanning electron microscope, in this article capital letters will be used, eg, SEM. 

A. J. Bevolo. Scanning Electron Microscopy. 1985, vol. 4, p. 1449. (Scanning Electron Microscopy, Inc. Elk Grove Village, IL) Thorough exposition of the principles and applications of reflected electron energy-loss microscopy (REELM) as well as a comparison to other techniques, such as SAM, EDS and SEM. 

An unusually extensive battery of experimental techniques was brought to bear on these comparisons of enantiomers with their racemic mixtures and of diastereomers with each other. A very sensitive Langmuir trough was constructed for the project, with temperature control from 15 to 40°C. In addition to the familiar force/area isotherms, which were used to compare all systems, measurements of surface potentials, surface shear viscosities, and dynamic suface tensions (for hysteresis only) were made on several systems with specially designed apparatus. Several microscopic techniques, epi-fluorescence optical microscopy, scanning tunneling microscopy, and electron microscopy, were applied to films of stearoylserine methyl ester, the most extensively investigated surfactant. 

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