Microorganisms Saccharomyces cerevisiae

Industrial Microorganisms Saccharomyces cerevisiae and other Yeasts... 

Microorganisms Saccharomyces cerevisiae, Staphylococcus aureus, Listeria monocytogenes [44,81]... 

Park C.B., Clark D.S. Sol-gel encapsulated enzyme arrays for high-throughput screening of hiocat-alytic activity. Biotechnol. Bioeng. 2002 78(2) 229-235 Pope E.H.A. Gel encapsulated microorganisms-saccharomyces-cerevisiae-silica-gel hiocomposites. J. Sol-Gel Sci. Technol. 1995 4(3) 225-229... 

Owing to diminishing fossil fuel reserves, alternative energy sources need to be renewable, sustainable, efficient, cost-effective, convenient and safe.1 In recent decades, microbial production of ethanol has been considered as an alternative fuel for the future because fossil fuels are depleting. Several microorganisms, including Clostridium sp. and yeast, the well-known ethanol producers Saccharomyces cerevisiae and Zymomonas mobilis, are suitable candidates to produce ethanol.2,3... 

There are two popular microorganisms that can produce high concentrations of alcohol. Their tolerance to high concentrations of ethanol and substrates are stated in the literature.3-5 The most common are Zymomonas mobilis and Saccharomyces cerevisiae. 

In this chapter, we shall focus on the molecular aspects of amino acid transport and its regulation in Saccharomyces cerevisiae. Kinetic, biochemical and genetic aspects of the amino acid transport systems of eukaryotic microorganisms have been reviewed earlier [7,8]. 

From this group of microorganisms, so far only Saccharomyces cerevisiae has been transformed to a poly(3HB) accumulating organism by expressing solely phflCRe in the cytoplasm ([137], Table 4). In contrast to E. coli and plants, which synthesize poly(3HB) only if a /3-ketothiolase and an acetoacetyl-CoA reductase... 

A variety of biotin-requiring microorganisms have been used to assay biotin Saccharomyces cerevisiae (H6), Lactobacillus casei (S2), Lactobacillus arabinosus (now L. plantarum ATCC No. 8014) (W14), Micrococcus sodonensis (Al), Neurospora crassa (H10), and Rhizobium tri-folii (W7). None have been applied successfully for assaying biotin in biologic fluids. Because the flagellate Ochromoms danica had a specific and sensitive biotin requirement (A2), it was utilized as a reagent for biotin in blood, serum, urine, brain, and liver tissue (B3b). 

A set of Saccharomyces cerevisiae reductases was screened in collaboration with J. D. Stewart s group (University of Florida). Itwas demonstrated that diketo ester la is accepted as substrate by at least three different NADP(H)-dependent reductases of this microorganism. Application of a cell-free system in preparative batches using enzyme-coupled coenzyme regeneration afforded (R)-2a with more than 99% enantiomeric excess [13]. 

Recently, it has been demonstrated that the yeast Saccharomyces cerevisiae (DHW S-3) can also be used for the (R)-selective reduction of the acetylsilane rac-48. By analogy with the bioconversions illustrated in Scheme 10, incubation of rac-48 with resting free cells of this microorganism yielded a 1 1 mixture of the corresponding diastereomeric (1-hydroxyethyl)silanes (SiR,CR)-49 and (SiS,CR)-5057. Under preparative conditions, the biotransformation products were isolated in 43% yield (relative to rac-48). The enantiomeric purities of the silanes (SiR,CR)-49 and (SiS,CR)-50 were >98% ee. 

You are going to cultivate yeast, Saccharomyces cerevisiae, by using a 10 m -fermenter your company already owns. You want to find out the amount of ethanol the fermenter can produce. Therefore, a chemostat study was carried out and the Monod kinetic parameters for the microorganism grown in the glucose medium at 30°C, pH 4.8, were found to be Ks = 0.0025 g/L and /imax = 0.25 h-1. The ethanol yield (YP/S) is 0.44 (g/g) and cell yield (Yx/S) is 0.019 (g/g). The inlet substrate concentration is 50 g/L-... 

Fungi (e.g. Cunninghamella sp., Aspergillus sp., Saccharomyces cerevisiae) are eukaryotic organisms, like mammals, and are the most commonly utilized microorganisms in biotransformation studies [31, 32]. The use of bacteria (prokaryotes) is limited mostly to actinomycetes that seem to contain an enzyme system very similar to that of fungi. Other bacteria (e.g. Pseudomonas, Escherichia coli) are used occasionally, but their usefulness is limited [26, 33]. 

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