Microorganisms naming

An alternative approach to the microbial deracemization of secondary alcohols is to use two different microorganisms with complementary stereoselectivity. Fantin et al. studied the stereoinversion of several secondary alcohols using the culture supernatants of two microorganisms, namely Bacillus stearothermophilus and Yarrowia lipolytica (Figure 5.18) [31]. The authors tested three main systems for deracemization. First, they used the supernatant from cultures of B. stearothermophilus, to which they added Y. lipolytica cells and the racemic alcohols. Secondly, they used the culture supernatant of Y. lipolytica and added B. stearothermophilus cells and the racemic alcohols. Finally, they resuspended the cells of both organisms in phosphate buffer and added the racemic alcohols. The best results were obtained in the first system with 6-penten-2-ol (26) (100% ee and 100% yield). The phosphate buffer system gave... 

Microorganism Name of Enzyme Target Sequence and Cleavage Sites ... 

Secondary metabolism is a form of differentiation, but cells grown in vitro are rapidly dividing, undifferentiated cells. Only at the end of the growth phase of batch-cultured cells may some form of differentiation occur, connected with the production of secondary metabolites. A plant produces a wide variety of secondary metabolites, all with different, mostly unknown functions. In in vitro cultured cells those compounds which defend the plant against microorganisms, namely, phytoalexins, are often easily formed. For example. Cinchona cell cultures produce large amounts of anthraquinones, but the alkaloids of interest, the quinolines, are produced in trace amounts only. Similarly Papaver cell cultures produce sanguinarine and closely related alkaloids, but no morphinane alkaloids. 

Why are bioflhns resistant to biocides It is interesting to see that most of authors with medical or microbiological background often use the term antibiotic and those with an engineering background prefer to use the term biocide in order to address the chanicals that kill the microorganisms, namely, bacteria. However, it must be noted that there is a rather delicate difference between these two terms, at least when they are applied to MIC cases. 

Pantothenic acid is found in extracts from nearly all plants, bacteria, and animals, and the name derives from the Greek pantos, meaning everywhere. It is required in the diet of all vertebrates, but some microorganisms produce it in the rumens of animals such as cattle and sheep. This vitamin is widely distributed in foods common to the human diet, and deficiencies are only observed in cases of severe malnutrition. The eminent German-born biochemist Fritz Lipmann was the first to show that a coenzyme was required to facilitate biological acetylation reactions. (The A in... 

Recently, recombinant biocatalysts obtained using Escherichia coli cells were designed for this process. The overexpression of all enzymes required for the process, namely, hydantoinase, carbamoylase, and hydantoin racemase from Arthrobacter sp. DSM 9771 was achieved. These cells were used for production of a-amino acids at the concentration of above 50 g 1 dry cell weight [37]. This is an excellent example presenting the power of biocatalysis with respect to classical catalysis, since a simultaneous use of three different biocatalysts originated from one microorganism can be easily achieved. 

This method depends on the fact that bacteria like all living organisms produce heat when they metabolize. Because of the small amount of heat produced, especially sensitive calorimetric devices are required hence the name microcalorimetry. The specimen to be evaluated is diluted with a nutrient medium and, if microorganisms are present and can metabolize, heat is produced and can be measured. An interesting offshoot of this technique is the fact that differing organisms produce different heat outputs and this may provide a means of identification. Microcalorimetry may enable organisms to be detected and possibly identified in 3 hours. 

The disinfectant is assessed on its overall performance, namely its ability to kill microorganisms, as judged by subculture recovery or lack of it and not by comparison with phenol, i.e. a disinfectant would pass or fail according to its performance. A use-dilution concentration of a disinfectant must pass the test at three replications. 

Woese chose the name archaebacteria because these microorganisms grow best under conditions which were probably found on the primeval Earth between 3.5 and 4 billion years ago hot boiling water and thermal vents, highly acidic environment, oxygen-free atmosphere and high salt concentrations. 

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