Methadone methanol

Note Tertiary amines and quaternary ammonium compounds yield stronger colors than primary amines [25]. The dipping solution can also be used as spray solution [44]. Other reagent compositions have also been reported in the literature (1, 3, 6, 12, 13, 15, 18, 21, 23, 41] In some cases the reagents have been made up in acetone [38, 39], methanol [14] or ethanol [37] and/or acidified with hydrochloric acid [3, 33, 37-40]. The concentrations of hexachloroplatinic(IV) acid have been in the range of 0.05 -0.4 those of potassium iodide between 0.5 and 24spray solution containing 2% potassium iodide and 0.23170 hexachloroplatinic(IV) acid hexahydrate in N-hydro-chloric acid is reported to yield the best coloration results with respect to detection sensitivity and color differentiation in the detection of morphine, codeine, quinine, methadone and cocaine [46]. Acidic reagent solutions have been recommended for benzodiazepines [10, 11]. Sulfones do not react [39]. 

FIGURE 1.32 Extraction-time profiles for promethazine (A), methadone (B), amphetamine (C), pethidine (D), and haloperidol (E) in 1 mL sample volume with 0, 5, and 50% methanol added to plasma sample.155 (Reproduced with permission from the Royal Society of Chemistry.)... 

Bouzas et al. [95] developed a quantitation method for the determination of drugs of abuse (opiates, amphetamine and derivatives, cocaine, methadone and metabolites) in serum by using online extraction coupled to LC-MS/MS. The online extraction procedure described consists of an extraction column and an analytical column, which were coupled online. A PPT procedure was performed with zinc sulfate an aliquot of 0.1 M zinc sulfate in methanol was added to the serum sample in a proportion of 2 1 (v/v) to serum. Analytes were extracted by a short pentafluorophenyl silica column and separated on a longer analytical column with the same stationary phase. Recoveries of all analytes were above 80 %. The proposed procedure by Matuszewski et al. [64] was used for the evaluation of matrix effect ME(%)=B/Ax 100, where ME is the matrix effect (suppression or enhancement) and B corresponds to peak areas for standards spiked after extraction into sample extracts and A to peak areas obtained in neat solution standards. Authors compared this method to offline SPE coupled with GC-MS and results showed that LC-MS/... 

Comparing SFE with other classical extraction methods, we have observed that, either the method is more efficient (basic/acid hydrolysis, extraction with methanol), or that it gives comparable results (enzymatic hydrolysis). The results obtained for cocaine and for methadone, although necessitating complementary studies, remain very encouraging. Lastly, if we take into account the ease of the method and its economic implication, this technique should be adopted without delay in laboratories involved in this type of analysis. 

Method. Apply 25 li1 of the reference solution to the first column of each of three plates. Divide the basic extract from the solvent extraction method, or the remainder of the column extract, equally between the three plates. Develop one plate in a tank containing 100 ml of methanol strong ammonia solution (100 1.5) (System TA, p. 167). Develop the other two plates in a tank containing ethyl acetate methanol strong ammonia solution (85 10 5) (System TE, p. 168). After development, remove Ihe plates from the tanks and dry under a stream of cold air until they no longer smell of ammonia. Avoid using a hot-airblower as methadone may volatilise. 

Methadone Dolophitie )— )4-%6%,z methamphetamine—44,66,68,134-5 methanol (methyl alcohoO-23 metheglin ( medicated mead)-307 M S i r e (ME raylERGonoviNE, i lysergic... 

Similar TLC behavior has been observed with (R,S)-6-[CH3- H]dimethylamino-4,4-diphenylhep-tane-3-one HCl ([ H]methadone [XI]) (silica gel Merck benzene/methanol/NH40H 85 15 0.65, v/v/ v) Rf [ H] = 0.58 Rp [ H] = 0.79. Reversed-phase LC showed baseline resolution, whereas gas-liquid chromatography afforded no separation. 

In an extremely powerful technique, Dams et al. [540] separated 18 opiates and their derivatives (morphine, codeine, naloxone, hydrocodone, papaverine, dextromethorphan, noscapine, bupremorphine, methadone, heroin, thebacone, ethylmor-phine, 6-monoacetylmorphine, acetyldihydrocodeine, acetylcodeine, normethadone, normorphine, norcodeine) with baseline resolution in <12 min This was accomplished on a fast LC phenyl column 53 x 7 mm (2 = 280 nm) using a simple 100/0—>50/50 (at 10min)—> 0/100 (at 12min hold Imin) (90/5/5 water/ methanol/acetonitrile with 50 mM ammonium acetate)/(50/50 methanol/ acetonitrile with 50 mM ammonium acetate) gradient. The mobile phase components were adjusted to pH pp 4.5 with formic acid prior to use. The phenyl column was chosen ova- the conventional Cg or C g columns because of the increased selective interaction with analytes containing the planar phenyl functional group. Detection limits from 50 to 450ng/mL (analyte dependent) were reported. 

Various alkaloids prepared in methanol were separated on KCig reversed-phase layers in a methanol-0.5 M NaCl (65 35) mobile phase (Whatman, TLC Technical Series, Volume 1). Spots were detected with the iodoplatinate reagent diluted with ethanol (2 1) just prior to use. The values of the separated alkaloids were as follows Valium, 0.16 methadone, 0.27 quinine, 0.37 phencyclidine, 0.49 meperidine, 0.52 Talwin, 0.57 cocaine, 0.58 codeine, 0.72 and morphine, 0.72. 

Metaproterenol Sulfate, USP Methadone Hydrochloride Oral Isopropyl alcohol and methanol Organic volatile impurities. Method IV < 467 > Alcohol content. Method II < 611 > P 2... 

Stock standard 1 Into a 50 mL volumetric flask pipette 0.5 mL each of fentanyl/norfentanyl (intermediate stock standard), tramadol, O-desmethyltramadol, and meperidine 5.0 mL each of normeperidine, methadone, and EDDP. Bring to volume with methanol and mix well. Final concentrations are 1,000 ng/ mL fentanyl and norfentanyl 10,000 ng/mL tramadol, O-desmethyltramadol, meperidine, and normeperidine and 100,000 ng/mL methadone and EDDP. 

---