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Membrane components procedure

Selection procedure. This is basically done by exposing the cell population to an antibody specific for an exposed cell membrane component (e.g. a specific protein, or an oligosaccharide determinant), and then adding complement to lyse all the cells that have bound enough antibody to trigger a cytotoxic effect. A selection procedure carried out on cells in suspension is described next. The same selection can also be applied to cell monolayers. [Pg.179]

In pervaporatlon, reverse osmosis, ultrafiltration and to a lesser extent microfiltration, both steric and chemical factors Influence permeation and separation. Thus, proper membrane material selection la Important. While the physical structure of the membrane is in large part a function of membrane preparation procedures, the chemical nature and, to some degree, the physical properties of the membrane are dependent upon the chemical nature of the membrane material. Thus, membrane material selection based on the chemical nature of the polymer and the solution components to be separated is feasible. [Pg.56]

Whether the approach outlined above Is used to select a pl oz i the membrane material or an approach based simply on polymer availability Is used. It would be convenient to evaluate the material for Its potential to accomplish the desired separation prior to forming a membrane. Once the membrane has been formed. It Is difficult to distinguish the Intrinsic properties of the polymer from the physical characteristics of the membrane such as porosity and asymmetry (that Is, properties which are governed In large part by membrane formation procedures). In this section a number of techniques for evaluating the material In particulate form as well as In the form of a dense film are discussed. Recall that the membrane Is to be used to separate a mixture of components A and B where B Is the bulk or major solution component and that Interaction between either solution component and the membrane material, M, may be Influenced by the presence of the other solution component. Thus, It would be difficult to evaluate a... [Pg.60]

Rationalization of the Verious Membrane Preparation Procedures. To discuss the formation of membrane structures it is useful to describe the concentration profiles of the casting solution components in the precipitating casting solution. The schematic diagram of Figure 1.21 shows the concentration profiles of polymer, solvent and precipitant at some intermediate time during the precipitation of a polymer film cast on a glass plate. [Pg.29]

As part of the discussion on the use of textile construction membranes, it should be made clear what happens at end-of-Ufe for materials used so far for building construction. Thermoplastic membrane components like PVC can be processed after each utilization period under certain conditions and be supplied for reuse in the raw material cycle. Leading European manufacturers of PVC/PES membranes and roofing sheets have united in order to recycle post-consumer waste, disused membranes and PVC materials (ref. Vinyl) in most modem plants with approved thermo-physical procedures. The output of this recycling process is then used for new products. An important factor in the operation of these and other environmental raw-material recovery procedures is logistics, which includes the materials being carefully prepared and sorted prior to delivery. The processing plants are potentially able to achieve a turnover of more than one ton per hour (ref. recovinyl). [Pg.65]

Following the procedures discussed in Section 3 images were acquired as shown in Fig. 11.1. In panel A ME-SIMS images of lymnaea Sta nalis brain tissue are shown and in panel B MetA-SIMS images of single neuroblastoma cells are shown. The plasma coating procedure in MetA SIMS enhances des-orption/ionization of membrane components such as lipids and sterols in SIMS imaging of tissues and cells. As shown... [Pg.205]

The PVC-based sensor membranes were prepared according the conventional procedure. The membrane components are as follows PVC (33 wt %), plasticizer bis(2-ethylhexyl) sebacate (DOS) or o-nitrophenyl octyl ether (o-NPOE) (66 wt %), and manganese(III)-tetraphenylporphyrin chloride (MnTPPCl) (1 wt %) were dissolved in freshly distilled tetrahydrofuran (THF). After 15 min of stirring, the cocktail was poured into a Petri dish. [Pg.259]


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