Enzymatic mechanisms

Digestion is the stepwise conversion of complex chemical and physical nutrients via mechanical, enzymatic, and physicochemical processes into molecular forms that can be absorbed from the GI tract. 

The extracellular matrix of mammalian tissue is composed of a complex mix of constitutive proteins. This matrix must be broken down to recover single cells effectively for culture and/or staining (1). Tissue dissociation and its affiliated problems were described and defined over 80 yr ago by Rous and Jones (2). More recent reviews (3,4) have revealed newer methods for creating single-cell suspensions. Numerous procedures exist for dissociating solid tumors. They are usually multistep procedures involving one or a combination of mechanical, enzymatic, or chemical manipulations. Ideally, the dissociation protocol is individualized for the tissue of interest and evaluated relative to both optimal and representative cell yield. 

Preparation Representativity Degradation Mechanical Enzymatic Concentration of LCC Linkages Solubility in NMR Solvents Comments/Recommendations... 

Rao, M. S., Tuyen, M. H., Stevens, W. F., and S. Chandrkrachang. 2001. Deproteination by mechanical, enzymatic and Lactobacillus treatment of shrimp waste for production of chitin. In T. Uragami, K. Kurita, and T. Fukamizo (eds.), Chitin and Chitosan Chitin and Chitosan in Life Science, pp. 301-304, Kodansha Scientific Ltd. Tokyo, Japan. 

Primary cell culture Removal of cells mechanically, enzymatically, or chemically from a host organism and their relocation to a culture vessel. 

In addition to conventional vegetal cellulose fibers (Figure 1 A), other forms of cellulose have been assessed in the last few years. The use of micro and nano-cellulose fibers, namely whiskers, obtained from a marine species (Samir, 2005), bacterial cellulose produced by some bacterial strains (Pecoraro, 2008) as well as micro- or nano-fibrillated cellulose prepared by mechanical, enzymatic or chemical treatments of the vegetal fibers (Nakagaito, 2004), for the development of high performance composite materials is attracting researchers from diverse fields (Dufresne, 2008 Lee, 2009), as the addition of very modest amounts of nano fibers leads to new composite materials with superior mechanical properties and new functionalities (Klemm, 2009) when compared with their conventional cellulose fibers counterparts. 

Mechanical, enzymatic, and chemical cell dissociation methods are currently used as passaging techniques for hPSCs. However, these methods are not well defined, which introduces variability into the system and may lead to cell damage. The enzymatic detachment of cells from dishes sometimes generates karyotype abnormalities. 

The bactericidal and enzymatic action of dyes, particularly of vinyl derivatives of 3,4,5-substituted thiazolium, for example, 45 (Scheme 70) (139), have been systematically studied to know more about the basic mechanisms involved (140). 

Km for an enzymatic reaction are of significant interest in the study of cellular chemistry. From equation 13.19 we see that Vmax provides a means for determining the rate constant 2- For enzymes that follow the mechanism shown in reaction 13.15, 2 is equivalent to the enzyme s turnover number, kcat- The turnover number is the maximum number of substrate molecules converted to product by a single active site on the enzyme, per unit time. Thus, the turnover number provides a direct indication of the catalytic efficiency of an enzyme s active site. The Michaelis constant, Km, is significant because it provides an estimate of the substrate s intracellular concentration. 

Biochemistry resulted from the early elucidation of the pathway of enzymatic conversion of glucose to ethanol by yeasts and its relation to carbohydrate metaboHsm in animals. The word enzyme means "in yeast," and the earfler word ferment has an obvious connection. Partly because of the importance of wine and related products and partly because yeasts are relatively easily studied, yeasts and fermentation were important in early scientific development and stiU figure widely in studies of biochemical mechanisms, genetic control, cell characteristics, etc. Fermentation yeast was the first eukaryote to have its genome elucidated. 

This resistance, inducible by low concentrations of dalbaheptides, is plasmid mediated and is transferable. Concomitant with the induction of resistance is the appearance or increased expression of a protein having a molecular weight of either 39,500 or 39,000. The enzymatic activity of this material has been postulated (112). Although the mechanism of resistance induction by dalbaheptides is unknown, different dalhabaheptides have different induction capacity. Vancomycin (39) is the most powerful inducer teicoplanin is a very weak inducer. 

Germicidal Properties. The germicidal activity of aqueous chlorine is attributed primarily to HOCl. Although the detailed mechanism by which HOCl kills bacteria and other microorganisms has not been estabUshed, sufficient experimental evidence has been obtained to suggest strongly that the mode of action involves penetration of the cell wall followed by reaction with the enzymatic system. The efficiency of destmction is affected by temperature, time of contact, pH, and type and concentration of organisms (177). 

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