Surface enhanced laser desorption ionization-mass spectrometry

Wright, G. L., Cazares, L. H., Leung, S.-M., Nasim, S., Adam, B.-L., Yip, T.-T., Schellhammer, P. F., Gong, L., and Vlahou, A. (2000). ProteinChip surface enhanced laser desorption/ionization (SELDI) mass spectrometry a novel protein biochip technology for detection of prostate cancer biomarkers in complex protein mixtures. Prostate Cancer and Prostatic Diseases 2, 264-276. 

Diamond DL, Zhang Y, Gaiger A, et al. Use of ProteinChip array surface enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) to identify thymosin beta-4, a differentially secreted protein from lymphoblastoid cell lines. J. Am. Soc. Mass Spectrom. 2003 14 760-765. 

Banks RE, Stanley AJ, Cairns DA, Barrett JH, Clarke P, Thompson D, et al. Influences of blood sample processing on low-molecular-weight proteome identified by surface-enhanced laser desorption/ionization mass spectrometry. Clin Chem 2005 51(9) 1637 1649. 

Guo J, Yang EC, Desouza L, Diehl G, Rodrigues MJ, Romaschin AD, et al. A strategy for high-resolution protein identification in surface-enhanced laser desorption/ionization mass spectrometry Calgranulin A and chaperonin 10 as protein markers for endometrial carcinoma. Proteomics 2005 5(7) 1953-1966. 

The Bik glycoprotein has an isoelectric point (p/) of 2.1 and is composed of a peptide and two glycoconjugate portions [30]. The predicted peptide sequence of Bik is helpful to understanding protein function. The peptide has Kunitz-binding domains I and II attached to the N-terminal peptide tail [31-33] (Fig. 2). Protein mass spectrometry by surface-enhanced laser desorption ionization (SELDI) in combination with detection by Bik antibodies has demonstrated that substantial variation exists in the Bik molecule [13,14]. 

RC-MS stems from the observation that mass spectra of peptides and proteins can be generated by laser activation of chromatographic surfaces placed on MALDI targets. The product application of RC-MS, called surface-enhanced laser desorption ionization-time of fiight (SELDI-TOF), refers to a mass spectrometry method for measuring native proteins adsorbed (retained) on various chemical or biochemical surfaces (Figure 4.11). Although the concept of laser desorption of intact proteins is well established, it has been commercially exploited only recently as a means... 

Amyloid P peptides in cerebrospinal fluid as profiled with surface enhanced laser desorption/ionization time-of-flight mass spectrometry evidence of novel biomarkers in Alzheimer s disease. 

Label-free optical techniqnes for detecting bound proteins on microarrays have been recently reviewed. The advantage of these methods over labeling methods is that the native form of the analyte is preserved. These methods include SPR, surface-enhanced laser desorption/ionization mass spectrometry (SELDI-MS), atomic force microscopy " and fiber-optic methods. 

Surface-Enhanced Laser Desorption/ionization Mass Spectrometry... 

Surface-enhanced laser/desorption ionization mass spectrometry Protein-Chip . See Tang, N., Tomatore, P, and Weinberger, S.R., Current developments in SELDI affinity technology. Mass Spectrom. Rev. 23a, 34-44,2004. 

At present, a variety of label-free detection strategies are available for biomedical research, such as surface plasmon resonance (SPR), CNTs, cantilevers, surface-enhanced laser desorption ionization (SELDI)-time of flight (TOF)-mass spectrometry (MS), microfluidic purification chips, immunosensors... 

Merchant, M. Weinberger, S.R. Recent Advancements in Surface-Enhanced Laser Desorption/Ionization-Time of Elight-Mass Spectrometry, Electrophoresis 21, 1164-1177 (2000). 

SURFACE-ENHANCED LASER-DESORPTION/IONIZATION TIME-OF-FLIGHT MASS SPECTROMETRY... 

Wright, G.L. Cazares, L.H. Leung, S.M. et al. ProteinChip Surface Enhanced Laser Desorption/ionization (SELDI) Mass Spectrometry A Novel Protein Biochip Technology for Detection of Prostate Cancer Biomarkers in Complex Protein Mixtures, Prostate Cancer and Prostatic Dis., 2, 264-276 (2000). 

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