Mass spectrometry , laboratory

The traditional mass spectrometry laboratory is equipped with top-of-the-line instrumentation, run by a mass spectrometrist for high-performance, nonroutine analyses. The use of mass spectrometry has recently... 

FAB and PD have been replaced by electrospray ionization (ESI) and matrix-assisted laser desorption ionization (MALDI) in the analytical mass spectrometry laboratory, because both of these newer techniques have a wider mass range of analysis and have lower detection limits. ESI and MALDI have become invaluable ionization techniques for nonvolatile components. This is particularly true for a wide range of biological molecules including proteins, peptides, nucleic acids, etc. Samples can be analyzed by ESI using either direct injection or introduction through liquid chromatography. 

Development of the method involved the installation of a system in an existing mass spectrometry laboratory and working with chemists for 3 months to determine specific needs and to develop a consistent, reliable procedure. The instrument was moved to an open-access laboratory and chemists were trained in its use. A key to making this approach a success is the fact that instrument downtime was kept to a minimum. Understandably, maintenance is done at off-peak times, and support mechanisms are put in place so problems are immediately addressed. Training and education was highlighted as a key factor for the successful implementation of this LC/MS system to optimize performance and to reduce the possibility of instrument contamination. 

CpWIr3(CO)n was prepared as previously described (2). Diphenylacetylene (Aldrich) was used as received. Preparative thin-layer chromatography was carried out on (20 x 20 cm) silica gel Si250F plates (J.T. Baker). Infrared spectra were recorded on a Perkin-Elmer 1750 FT-IR spectrophotometer. H NMR (360 MHz) spectra were obtained on a Nicolet NT-360 spectrometer. Fast atom bombardment (FAB) mass spectra were obtained by the staff of the Mass Spectrometry Laboratory of the School of Chemical Sciences on a VG ZAB-SE mass spectrometer, using a matrix of dithioerythritol/dithiothieitol calculated masses are referenced to 184W and 193Ir. Elemental analyses were performed by the School Microanalytical Laboratory. 

Morand K, Burt TM, Dobson RLM, Wilson LJ, Development of a mass spectrometry laboratory for support of pharmaceutical combinatorial chemistry programs, Proc. 45th ASMS Conf, p. 1261, 1997. 

Francesco Giorgianni, Charles B. stout Neuroscience Mass Spectrometry Laboratory, The University of Tennessee Health Science Center, Memphis, Tennessee... 

ThoMSon Mass Spectrometry Laboratory, Institute of Chemistry, State University of... 

Marcos N. Eberlin ThoMSon Mass Spectrometry Laboratory, Institute of Chemistry, State University of Campinas, 13084 971 Campinas, SP, Brazil. Fax +55 19 3521 3073 e-mail eberlin iqm.unicamp.br... 

Because use of mass spectrometry by chemists has increased greatly, most U.S. chemists have access to mass spectrometry facilities at their own institutions to confirm synthesis and support structure elucidations. Heavily used national centers provide more expensive instrumentation and more complex experiments. Most notably, a section of the National High Magnetic Field Laboratory at Florida State University provides state-of-the-art Fourier transform ion cyclotron resonance mass spectrometry. The NSF Arizona Accelerator Mass Spectrometry Laboratory is used primarily to provide radiocarbon measurements. NIH funds a number of national mass spectrometry centers to support biomedical research, including those at Boston University and the Pacific Northwest National Laboratory. 

At Rockefeller University, I also had the opportunity to know Professor Prank H. Pield, director of the mass spectrometry laboratory. Earlier, Dr. Pield, in collaboration with Joe Pranklin, had developed the first ionization technique for mass spectrometry. Dr. Pield was helping Professor Tatum with the identification of chemical(s) emitted into the gas phase by a slow-growing morphological mutant of Neurospora. An exposure of this gaseous emission to the wild-type strain made it grow slowly like the mutant. This chemical, however, remained elusive to identification by mass spectrometry. 

Johns Hopkins University School of Medicine, Department of Pharmacology Molecular Sciences, Middle Atlantic Mass Spectrometry Laboratory, Baltimore MD 21205, USA... 

Moon Ion- and neutral mass spectrometry UV spectroscopy Mass spectrometry, laboratory (Apollo Luna samples, lunar meteorites) He and Ar in lunar exosphere on ground, Ar/ °Ar. Stem 1999b. Upper limit for Kr, Xe from orbit. Feldman and Morrison 1991. Ar-Ar ages, exposure ages, solar wind etc. Turner 1970 Culler et al. 2000 Wieler 1998. 

Mars Mass spectrometry (Viking) EUV spectroscopy Mass spectrometry, laboratory (Martian meteorites) Atmosphere. Nier and McElroy 1977 Owenetal. 1977. He in atmosphere. Krasnopolsky et al. 1994. Martian atmosphere, cmst, mantle. Bogard et al. 1984 Becker and Pepin 1984 Swindle 2002b. 

Asteroids Mass spectrometry, laboratory (meteorites) Ott 2002 Swindle 2002a. 

Interplanetary dust particles, micrometeorites Mass spectrometry, laboratory Dust from asteroids comets, collected in near Earth space, ice, sediments. Nier and Schlutter 1992 Olinger et al. 1990. ... 

In a modem, state-of-the-art mass spectrometry laboratory, the determination of the molecular formula of an unknown substance is done using exact masses. The spectroscopist must be careful, however, as the method cannot be used if the molecular ion peak is very weak or absent. 

Ease of use. Indeed, in most cases, users do not have to be specifically trained in a mass spectrometry laboratory. 

Bogialli S, D Ascenzo G, Di Corcia A, Lagana A, Tramontana G, Simple assay for monitoring seven qninolone antibacterials in eggs Extraction with hot water and hquid chromatography coupled to tandem mass spectrometry Laboratory validation in hne with the Enropean Union Commission Decision 657/2002/EC, J. Chromatogr. A 2009 1216 794-800. 

National Institutes of Health, United States Public Health Service (Research Grants GM 19978 and GM 20441), by the United States Army Research Office (Research Grant GM DAHC04-75-6-0179) and the gas chromatography-mass spectrometry laboratory supported by the University of Minnesota Agricultural Experiment Station, Scientific Journal Series No. 10,738, Agricultural Experiment Station, University of Minnesota, St. Paul, Minn. 55108. 

University of Liege, Mass Spectrometry Laboratory, Biological Organic Analytical Chemistry, Alice du 6 aout, B6c, B-4000 Liege (Sart-Tilman), Belgium... 

Chemical element B has two stable isotopes, B and "B, nuclei of which differ by one neutron. In the universe, "B/ B natural abundance ratio is estimated as 4.05 0.10 (Viola 1991). This value coincides with the natural isotopic composition of boron, 19.85% B+80.15% "B, determined in floating-zone-melted P-rhombohedral boron by the glow-discharge mass-spectrometry laboratory method (Nogi et al. 2000). Since boron and its compounds have found a variety of applications, the elemental and isotopic analyses of boron become important steps of an investigation with special challenges (Balaram 2011). 

It is argued in this paper that routine, rapid generation of data in an open-access mass spectrometry laboratory can make the data acquired timely and thus increase its relevance. To achieve this goal, it was important to simplify both experimental protocols and instrument operation. 

ThoMSon Mass Spectrometry Laboratory University of Campinas Campinas, Brazil... 

CH2CI2 and aniline were obtained from Fischer Scientific Co. and distilled from CaH2 (Aldrich Chemical Co.). 3,4,9,10-perylenetetracarboxylic dianhydride, 2-chloro-5-nitrobenzoic acid, sodium borohydride, copper (II) acetyl-acetonate, trifiuoroacetic acid (TFA), tetrabutylammonium hexafluorophosphate (TBAPF6), dimethylacetamide (DMA), and N-phenyl-l,4-phenylenediamine were purchased from Aldrich Chemical Co. and used as received. Quinoline and triethylamine (TEA) (Aldrich Chemical Co.) were distilled in vacuo. IR spectra were obtained on a Bruker Equinox 55 Spectrometer. NMR spectra were obtained on a Bruker 250 multiprobe spectrometer. Elemental andyses were performed by Atlantic Microlabs. Mass spectrometry data were provided by the Auburn Mass Spectrometry Laboratory. 

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