Mass spectrometry, Fourier transform ion

Structural characterization of proteins and peptides using quadrupole ion trap mass spectrometry, Fourier transform-ion cyclotron resonance (FT-ICR) mass spectrometry, and traveling wave ion mobility mass spectrometry... 

Part 2. Ion Conformation and Structure presents discussions of structural characterization of proteins and peptides using quadrupole ion trap mass spectrometry, Fourier transform ion cyclotron resonance mass spectrometry, and the novel method known as traveling wave ion mobility mass spectrometry. In addition to the observation of collective fluctuations of the molecular substructures within biomolecules, the organization of atoms in small ion clusters is investigated using electron diffraction. 

Schaub, T. M., Hendrickson, C. L., Horning, S., Quinn, J. P., Senko, M. W., Marshall, A. G. (2008) High-performance mass spectrometry Fourier transform ion cyclotron resonance at 14.5 tesla. Anal Chem, 80, 3985-3990. 

Fig. 1 Infrared photodissociation action spectra in the fingerprint infrared region Irom fairly early in the modem evolution of spectroscopy of biologically interesting complex ions. These spectra illustrate the use of electrospray ion production, ion trapping mass spectrometry (Fourier-transform ion cyclotron resonance in these examples) and the FELIX free electron laser light source. Lower spectrum originally derived from [10, 11] middle spectrum frran [12] upper spectrum from [13]. Figure reproduced with permission from [12]...

Schaub, T.M. Hendrickson, C.L. Homing, S. Quinn, J.P. Senko, M.W. Marshall, A.G. High-Performance Mass Spectrometry Fourier Transform Ion Cyclotron Resonance at 14.5 Tesla. Anal. Chem. 2008, 80, 3985-3990. 

Perfluoroethylenes have been characterized by desorption chemical ionization and tandem mass spectrometry Fourier transform ion cyclotron resonance mass spectroscopy has also been applied to the identification of polymers, eg. polyethylene glycols. Comparative complimentary plasma desorption mass spectrometry/secondary ion mass spectrometry has been applied to the identification of oligomers of various polymers including polyethylene glycol, polytetrafluoroethylene, polycarbonate, polyacrylates, polyethylene terephthalate and siloxanes. ... 

The advent of techniques that enable the study of fast reactions in the gas phase, such as ion cyclotron resonance (ICR) spectrometry, Fourier-transform ion cyclotron resonance spectrometry (FT-ICR) and high pressure mass spectrometry (HPMS), allowed the measurement of the gas-phase proton affinities for strong bases84-86 as well as for... 

Silver ion TLC offers an effective means of fractionation of Upid mixtures into distinct fractions differing in the number of double bonds. It is often used to simplify the further examination with gas chromatography, GC-mass spectrometry, Fourier transform infrared, and so forth. Ag TLC serves also as an enrichment procedure for minor components and allows for more accurate estimation of their content and identity. Quantitative procedures have been developed for the determination of fatty acids and triacylglycerols by using Ag TLC and densitometry. 

Shi, S.D. Hendrickson, C.L. Marshall, A.G. Siegel, M.M. Kong, R Carter, G.T. Structural Validation of Saccharomicins by High Resolution and High Mass Accuracy Fourier Transform-Ion Cyclotron Resonance-Mass Spectrometry and Infrared Multiphoton Dissociation Tandem Mass Spectrometry, J. Am. Soc. Mass Spectrom. 10(12), 1285-1290(1999). 

Munson and Field reported in 1966 on a technique of ionizing molecules by gas phase ion-molecule reactions, which they called chemical ionization (Cl). In this way, break-up of the molecules can be greatly reduced or even avoided. Thus, measured ion currents can be correlated with the densities of the respective parent neutral compounds, allowing for on-line monitoring of rather complex gas mixtures. The fundamental principles of gas phase ion chemistry on which Cl is based, as well as the instrumentation for Cl, have been reviewed in great detail by Harrison." The wide variety of Cl methods that has been developed includes Medium Pressure Mass Spectrometry, Fourier Transform Mass Spectrometry, Quadrupole Ion Trap Mass Spectrometry, Pulsed Positive Ion-Negative Ion Chemical Ionization, and Atmospheric Pressure Ionization Mass Spectrometry (API-MS). Of these, API-MS has developed into a very reliable and widely used technique for analysis of VOCs in flavor release studies and human breath. A variety of API-MS applications in these fields of research has been described in a recent volume by Roberts and Taylor. ... 

Shi, S.D.-H., Hendrickson, C.L., Marshall, A.G., Siegel, M.M., Kong, F. and Carter, C.T. (1999) Structural validation of saccharomicins by high resolution and high mass accuracy Fourier transform-ion cyclotron resonance-mass spectrometry and Infrared multiphoton dissociation tandem mass spectrometry. Journal of the American Society for Mass Spectrometry 1 0, 1285-1290... 

Fourier-trans-form mass spectrometry Fourier-transform mass spectrometry takes advantage of ion-cyclotron resonance to select and detect ions 163,234,235... 

Investigation of interfacial effects and interactions between biocompounds and transducer surfaces at the molecular and atomic levels by exploiting new methods of surface analysis (e,g scanning microscopy techniques, fast-atomic-bombardment mass spectrometry, laser-assisted mass spectrometry, time-of-flight secondary ion mass spectrometry, Fourier-transform infrared spectrometry, ellipsometry. X-ray photoelectron spectroscopy, and electron microscopy)... 

Vartanian V H, Anderson J S and Laude D A 1995 Advances in trapped ion cells for Fourier transform ion cyclotron resonance mass spectrometry Mass Spec. Rev. 41 1-19... 

FTICR. Fourier-transform ion cyclotron resonance GC/IRMS. gas chromatography isotope ratio mass spectrometry... 

Jensen P.K., Pasa-Tolic L., Anderson G. A., Horner J. A., Lipton M.S., Bruce J.E., and Smith R.D., Probing proteomes using capillary isolectric focusing-elec-trospray ionization Fourier transform ion cyclotron resonance mass spectrometry, Anal. Chem. 71, 2076, 1999. 

Resolution does not affect the accuracy of the individual accurate mass measurements when no separation problem exists. When performing accurate mass measurements on a given component in a mixture, it may be necessary to raise the resolution of the mass spectrometer wherever possible. Atomic composition mass spectrometry (AC-MS) is a powerful technique for chemical structure identification or confirmation, which requires double-focusing magnetic, Fourier-transform ion-cyclotron resonance (FTICR) or else ToF-MS spectrometers, and use of a suitable reference material. The most common reference materials for accurate mass measurements are perfluorokerosene (PFK), perfluorotetrabutylamine (PFTBA) and decafluorotriph-enylphosphine (DFTPP). One of the difficulties of high-mass MS is the lack of suitable calibration standards. Reference inlets to the ion source facilitate exact mass measurement. When appropriately calibrated, ToF mass... 

In mass spectrometers, ions are analysed according to the ml7. (mass-to-charge) value and not to the mass. While there are many possible combinations of technologies associated with a mass-spectrometry experiment, relatively few forms of mass analysis predominate. They include linear multipoles, such as the quadrupole mass filter, time-of-flight mass spectrometry, ion trapping forms of mass spectrometry, including the quadrupole ion trap and Fourier-transform ion-cyclotron resonance, and sector mass spectrometry. Hybrid instruments intend to combine the strengths of the component analysers. 

Fourier-Transform Ion-Cyclotron Resonance Mass Spectrometry... 

B. Asamoto (ed.), FT-ICR/MS Applications of Fourier Transform Ion Cyclotron Resonance Mass Spectrometry, VCH Publishers, New York, NY (1991). 

Fourier transform ion cyclotron resonance mass spectrometry... 

CsFeo.72Agi.28Te2,1053 and Cs2Ag2ZrTe4. The latter has a structure that comprises 2D slabs of Ag- and Zr-centered tetrahedral separated by Cs+ cations.1054 Gas-phase silver chalcogenide ions of the type [Ag2 i E ] (E = S, Se, Te) with < 14 have been investigated by laser-ablation Fourier transform ion cyclotron resonance mass spectrometry.1055... 

Currently PCR and mass spectrometry are performed by two separate instruments. However, there is no reason why PCR followed by simple automated cleanup and mass spectrometry cannot be incorporated into a single integrated instrument. Essentially every configuration of the modern ESI mass spectrometer has been used successfully for the analysis of PCR products, from the highest to the lowest resolution involving. Fourier transform ion cyclotron resonance (FTICR), triple quadrupole, quadrupole-time of flight (Q-TOF), and ion trap.22-24 MS discriminates between two structurally related PCR products by MW difference. Mass accuracy is needed to differentiate the... 

Wunschel, D. S. Fox, K. F. Bruce, J. E. Muddiman, D. C. Smith, R. D. Analysis of double-stranded polymerase chain reaction products from the Bacillus cereus group by electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry. Rapid Comm. Mass Spectrom 1996,10, 29-35. 

Leinweber, F.C., Schmid, D.G., Lubda, D., Wiesmuller, K., Jung, G., Tallarek, U. (2003). Silica-based monoliths for rapid peptide screening by capillary hquid chromatography hyphenated with electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry. Rapid Commun. Mass. Spectrom. 17, 1180-1188. 

Kalkhof, S. et al. (2005a) Chemical cross-linking and high-performance fourier transform ion cyclotron resonance mass spectrometry for protein interaction analysis Application to a calmodulin/target peptide complex. Anal. Chem. 77, 495-503. 

Multiple mass analyzers exist that can perform tandem mass spectrometry. Some use a tandem-in-space configuration, such as the triple quadrupole mass analyzers illustrated (Fig.3.9). Others use a tandem-in-time configuration and include instruments such as ion-traps (ITMS) and Fourier transform ion cyclotron resonance mass spectrometry (FTICRMS or FTMS). A triple quadrupole mass spectrometer can only perform the tandem process once for an isolated precursor ion (e.g., MS/MS), but trapping or tandem-in-time instruments can perform repetitive tandem mass spectrometry (MS ), thus adding n 1 degrees of structural characterization and elucidation. When an ion-trap is combined with HPLC and photodiode array detection, the net result is a profiling tool that is a powerful tool for both metabolite profiling and metabolite identification. 

Resolutions in the range of 10,000 to 20,000 are achievable with modem time-of-flight mass spectrometry (TOFMS). Fourier transform ion cyclotron resonance mass spectrometry (FTICRMS) is more costly but capable of resolutions exceeding 100,000. Resolutions exceeding 10,000 can provide low to sub parts-per-million mass accuracies. One ppm is equivalent to a mass accuracy of 0.001 for a molecular weight of 1,000 Da. 

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