Target peptides

Ikura, M., et al. Solution structure of a calmodulin-target peptide complex by multidimensional NMR. Science 256 632-638, 1992. 

Hoen P.A. C., Prince P., Van der Bilt E., Valentijn A. R., Meeuwenoord N.J., Princen H., Bijsterbosch M.K., van der Marel G.A., van Boom J.H., van BerkelT.J.C. Design of a targeted peptide nucleic acid prodrug to inhibit hepatic human microsomal triglyceride transfer protein expression in hepato-cytes. Bioconjug. Chem. 2002 13 295-302. 

Muratovska a., Lightowlers R. N., Taylor R.W., Turnbull D.M., Smith R.A. J., WiLCE J.A., Martin S.W., Murphy M.P. Targeting peptide nucleic acid (PNA) oligomers to mitochondria within cells by conjugation to lipophilic cations implications for mitochondrial DNA replication, expression and disease. Nucleic Acids Res. 2001 29 1852-1863. 

Conotoxins Targeted Peptide Ligands from Snail... 

OLIVERA ET AL. Conotojdns Targeted Peptide Ligands from Snail Venoms 257... 

This article summarises the general characteristics of a novel antibacterial target, peptide deformylase (PDF) and reviews the design, structure-activity relationships (SAR) and properties of known PDF inhibitors, including pre-clinical and clinical data for the most advanced members of this class. 

This is traditionally justified since it removes the bulky fluorescent dye from the bioactive sequence. Nonacidic cleavage conditions in the release of targeted peptide from the resin is also a strategy when FTIC is used in solid phase peptide synthesis. 

Rajotte D, Ruoslahti E. Membrane dipeptidase is the receptor for a lung-targeting peptide identified by in vivo phage display. J Biol Chem 1999 274 11593-11598. 

Matsuo K, Yoshikawa T, Oda A et al (2007) Efficient generation of antigen-specific cellular immunity by vaccination with poly(y-glutamic acid) nanoparticles entrapping endoplasmic reticulum-targeted peptides. Biochem Biophys Res Commun 362 1069-1072... 

Kalkhof, S. et al. (2005a) Chemical cross-linking and high-performance fourier transform ion cyclotron resonance mass spectrometry for protein interaction analysis Application to a calmodulin/target peptide complex. Anal. Chem. 77, 495-503. 

Fig. 1.2 Protein blot analysis of human therapeutic protease inhibitor (HTPI) produced in alfalfa cell cultures using different promoters and subcellular targeting peptides as shown. Equal amounts of total soluble proteins from cell cultures were separated by sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) and blotted onto a polyvinyldifluoride (PVDF) membrane. Monoclonal anti-HTPI IgGs were used for detection.

Fig. 1.3 Prediction of the most appropriate subcellular targeting strategies by agroinfiltration. The levels of an industrial enzyme (IE) are shown in agroinfiltrated and transgenic alfalfa leaves using different subcellular targeting peptides. Equal amounts of total soluble leaf proteins were separated by SDS-PAGE and blotted onto a PVDF membrane. Polyclonal anti-IE IgGs were used for detection.

Terlau, H. and Olivera, B. M. Conus venoms a rich source of novel ion channel-targeted peptides. Physiol. Rev. 84 41-68, 2004. 

Pd°-catalyzed deprotection of S-palmitoylated dipeptide 9 yielded the corresponding selectively deprotected peptide 10. Condensation of 10 with farnesylated pentapeptide 15, which was readily accessible via the Aloe methodology1201 as depicted in Scheme 10 and alternatively using AcOZ1111 as the protecting group, resulted in the formation of target peptide 16.17251... 

Figure 11.9 (Left) The EF hand helix-loop-helix motif (centre) rat testes calmodulin. The globular domains each have two Ca2+-binding sites, indicated by white spheres, connected by a seven-turn a-helix (right) two views of the (Ca2+)4 fruit fly calmodulin in complex with its 26-residue target peptide from rabbit skeletal muscle myosin light chain kinase, ((left, centre) From Voet and Voet, 2004. Reproduced with permission from John Wiley Sons., Inc. and (right) Carafoli, 2002. Copyright (2002) National Academy of Sciences, USA.)...

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