Ion-mobility mass spectrometry

In addition to this enabling advance for direct tissue lipid analysis, the significance of IM-MS in lipidomics is numerous. First, separation of isomers, isobars, and con-formers is rendered possible with the addition of IM cells to mass spectrometers [97], which allows identification of novel lipid classes and species in a high-throughput manner. Moreover, analysis of chiral isomers could be achieved by the introduction of chiral reagents into an ion-mobility cell as demonstrated in other studies [98]. Finally, the duty cycle of IM-MS is short relative to LC separations and can thus be coupled to such techniques to form 3D modalities such as LC-IM-MS [102]. A recent review paper [103], which extensively discusses the principles of IM-MS technology and its applications for lipidomics, should be consulted for the readers who are interested in this area of research. 

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HRTG High-resolution thermogravimetry IMMS Ion mobility mass spectrometry... 

With a focus on trace forensic detection of explosives, especially for use in counterterrorism and to counter narcotics investigations, Fetterolf et al. [75] evaluated the use of ion mobility-mass spectrometry for explosives determinations. In this, explosives residues were collected on a membrane filter by a special attachment on a household vacuum cleaner. Although subsequent thermal desorption and analysis required only 5 s, fimits of detection for most common explosives were as low as 200 pg. The persistence of explosives on hands and transfer to other surfaces were also examined as were post-blast residues of NG on fragments of improvised explosive devices constructed with double-based smokeless powder. Finally, postblast residue from C-4, Semtex, and other explosives was found by IMS analyses on items of forensic and evidentiary value. These few out of many examples demonstrate that mobihty spectrometers are well suited tools for laboratory and on-site investigations, before and after the use of explosives. 

Ells, B., Froese, K., Hrudey, S. E., Purves, R. W., Guevremont, R., and Barnett, D. A. (2000). Detection of microcystins using electrospray ionization high-held asymmetric waveform ion mobility mass spectrometry/mass spectrometry. Rapid Commun. Mass Spectrom. 14 1538-1542. 

McCooeye, M., and Mester, Z. (2006). Comparison of flow injection analysis electrospray mass spectrometry and tandem mass spectrometry and electrospray high-field asymmetric waveform ion mobility mass spectrometry and tandem mass spectrometry for the determination of underivatized amino acids. Rapid Commun. Mass Spectrom. 20 1801-1808. 

McLean, J. A., Ridenour, W. B., and Caprioli, R. M. (2007). Profiling and imaging of tissues by imaging ion mobility-mass spectrometry. J. Mass Spectrom. 42 1099-1105. 

Venne, K., Bonneil, E., Eng, K., and Thibault, P. (2005). Improvement in peptide detection for proteomics analyses using nano LC-MS and high-held asymmetry waveform ion mobility mass spectrometry. Anal. Chem. 77 2176-2186. 

Thalassinos, K., et al. (2004). Ion mobility mass spectrometry of proteins in a modified commercial mass spectrometer. Int. J. Mass Spectrom. 236(1 —3) 55—63. 

Dwivedi P, Hill HH Jr (2008) A rapid analytical method for hair analysis using ambient pressure ion mobility mass spectrometry with electrospray ionization (ESI-IMMS). Int J Ion Mobil Spec 11 61-69... 

Stauber J, MacAleese L, Franck J, Claude E, Snel M, Kaletas B, Wiel I, Wisztorski M, Fournier I, Fournier I (2010) On-tissue protein identification and imaging by MALDI-ion mobility mass spectrometry. J Am Soc Mass Spectrom 20 338-347. doi 10.1016/j. jasms.2009.09.016... 

Fig. 1. Schematic outline of the basic ion mobility-mass spectrometry setup using mass selection before (MSI) and after (MS2) the drift tube...

Ion mobility mass spectrometry separates ions by their cross-sectional area as well as mass. This provides the opportunity to study conformational changes to a molecule s structure. 

W. E. Steiner, B. El. Clowers, L. M. Matz, W. F. Siems and El. El. Elill Jr., Rapid screening of aqueous chemical warfare agent degradation products ambient pressure ion mobility mass spectrometry. Anal. Chem. 74,4343 (2002). 

Tempez, A., Ugarov, M., Egan, T., Schultz, J. A., Novikov, A., Della-Negra, S., Lebeyec, Y, Pautrat, M., Caroff, M., Smentkowski, V. S., Wang, H. Y. J., Jackson, S. N., and Woods, A. S., Matrix implanted laser desorption ionization (MILDI) combined with ion mobility-mass spectrometry for bio-surface analysis. Journal ofProteome Research, 4, 540-545, 2005. 

Williams, J.P. Bugarcic, T. Habtemariam, A. Giles, K. Campuzano, I. Rodger, P.M. Sadler, P.J., Isomer separation and gas-phase configurations of organoruthenium anticancer complexes ion mobility mass spectrometry and modeling, J. Am. Soc. Mass Spectrom. 2009, 20,1119-1122. 

Mobility measurements and technology for ion characterization under vacuum or low pressure have become a feature of IMS, largely associated with ion mobility-mass spectrometry (IM-MS) applications for studies in biochemistry, biology, and... 

FIGURE 9.1 Mobility-mass correlation curves (trend lines). (From McLean et al Ion mobility-mass spectrometry a new paradigm for proteomics, Int. J. Mass Spectrom. 2005 240, 301-315. With permission.)... 

ATMOSPHERIC PRESSURE DRIFT TUBE ION MOBILITY-MASS SPECTROMETRY... 

FIGURE 9.10 Two-dimensional direct infusion ESI ion mobility MS spectrum of E. coli culture producing over 1,000 metabolite peaks. (Reprinted fromDwivedi et al.. Metabolic profiling by ion mobility-mass spectrometry (IMMS), Metabolomics 2007, 21, 1115-1122. With permission.)... 

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