Mammalian cells, adhesion

Low SP, Williams KA, Canham LT, Voelcker NH (2006) Evaluation of mammalian cell adhesion on surface-modified porous silicon. Biomaterials 27 4538 Makara VA, Klyui NI, Rozhin AG, Litovchenko VG, Piryatinskii YP, Kometa OB (2003) Porous silicon photoluminescence modification by surface treatments and impregnation of carbon based nanoclusters. Phys Status Solidi A-Appl Res 197 355 Makila E, Bimbo LM, Kaasalainen M, Herranz B, Airaksinen AJ, Heinonen M, Kukk E, Hirvonen J, Santos HA, Salonen J (2012) Amine modification of thermally carbonized porous silicon with silane coupling. Langmuir 28 14045... 

Stenger DA, Georger JH, Dulcey CS, Hickman JJ, Rudolph AS, Nielsen TB, McCort SM, Calved JM. (1992) Coplanar molecular assemblies of amino- and perfluorinated alkylsilanes Characterization and geometric definition of mammalian cell adhesion and... 

Fig. 4.1 Mammalian cell adhesion onto a biomaterial. a Initial contact between the cell and the biomaterial conditioned with serum proteins, b Bonding between ceU surface receptors and protein adhesion Ugands. c CeU cytoskeletal reorganization with progressive ceU spreading onto the biomaterial surface. (Adapted from Ratner et al. [15])...

An endo-a-sialidase (EC 3.2.1.129 Cabezas, 1991) exists in bacteriophages, which hydrolyzes a2,8 bonds within the polysialyl chains of bacterial colominic acid and mammalian cell adhesion molecules (Long et al., 1993). 

Kaibuchi K, Amano M. Regulation of the cytoskeleton and cell adhesion by the Rho family GTPases in mammalian cells. Annu Rev Biochem 1999 68 459-486. 

Lopez GP, Albers MW, Schreiber SL, Carroll R, Peralta E, Whitesides GM (1993) Convenient methods for patterning the adhesion of mammalian cells to surfaces using self-assembled monolayers of alkanethiolates on gold. J Am Chem Soc 115 5877-5878... 

Extracellular ligands (hormones, neurotrophins, carrier protein, adhesion molecules, small molecules, etc.) will bind to specific transmembrane receptors. This binding of specific ligand induces the concentration of the receptor in coated pits and internalization via clathrin-coated vesicles. One of the best studied and characterized examples of RME is the internalization of cholesterol by mammalian cells [69]. In the nervous system, there are a plethora of different membrane receptors that bind extracellular molecules, including neurotrophins, hormones and other cell modulators, being the best studied examples. This type of clathrin-mediated endocytosis is an amazingly efficient process, capable of concentrating... 

Unnecessary animal testing can be avoided by relying on in vitro studies as the first stage acute toxicity and cytocompatibility test for injectable materials (Pearce et al., 2007). Many researchers have utilized in vitro studies to screen the cellular response to CNTs (Raja et al., 2007) and to investigate the various CNT-mammalian cell interactions such as oxidative stress (Manna et al., 2005 Shvedova et al., 2003, 2007), antiproliferative effects (Cui et al., 2005 Garibaldi et al., 2006), decreased cell adhesion (Cui et al., 2005), apoptosis (Bottini et al., 2006 Cui et al., 2005 Jia et al., 2005), and necrosis (Jia et al., 2005). A summary of these in vitro studies are presented in Table 12.1. 

Dewez JL, Lhoest JB, Detrait E et al. (1998) Adhesion of mammalian cells to polymer surfaces from physical chemistry of surfaces to selective adhesion on defined patterns. Biomaterials 19 1441-1445... 

R. Feizi, Oligosaccharides that mediate mammalian cell-cell adhesion, Curr. Opin. Struct. Biol. 5 701 (1993), and references therein. 

Another method to retain cells is to trap the cells inside a polymer matrix. For instance, mammalian cells were entrapped in PEG-based hydrogel fabricated in glass-Si chips (see Figure 8.19). The cells included murine 3T3 fibroblasts and SV-40- transformed murine cells (i.e., hepatocytes, macrophages). A cell adhesion... 

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