Adhesion of mammalian cells

Lopez GP, Albers MW, Schreiber SL, Carroll R, Peralta E, Whitesides GM (1993) Convenient methods for patterning the adhesion of mammalian cells to surfaces using self-assembled monolayers of alkanethiolates on gold. J Am Chem Soc 115 5877-5878... 

Dewez JL, Lhoest JB, Detrait E et al. (1998) Adhesion of mammalian cells to polymer surfaces from physical chemistry of surfaces to selective adhesion on defined patterns. Biomaterials 19 1441-1445... 

Massia, S.P. Hubbell, J.A. Covalently attached GRGD on polymer surfaces promotes biospecific adhesion of mammalian cells. Ann. NY. Acad. Sci. 1990, 589, 261-270. 

It is known that more highly conducting nanodomains exist within the essentially amorphous ICP host structure and that electrochemical switching speeds can be extremely rapid in nanowires composed of these materials. It is also known that control of nanotopography in other biomaterials can have a profound effect on the adhesion of mammalian cells. Given the explosion of activity in the area of ICP nanostructures, there is no doubt that these enhanced properties should translate into more effective biomolecular sensors and actuators. 

Dexter, S.J., Pearson, R.G., Davies, M.C., Camara, M., Shakesheff, K.M. A comparison of the adhesion of mammalian cells and Staphylococcus epidermidis on fibronectin-modified polymer surfaces. J. Biomed. Mater. Res. 56(2), 222-227 (2001)... 

The growth rate of mammalian cells in the presence of Cell-Tak adhesive was assayed to evaluate any potential adverse effects caused by this protein. Bovine corneal endothelial cell (BCE) stocks were grown to confluency in MEM plus 15% calf serum, trypsinized, and washed several times by centrifugation on MEM. Suspensions (5 x 104 cells/mL) were seeded onto untreated 35-mm dishes (control) and dishes with Cell-Tak protein (5 fig/cm2) in MEM with 15% calf serum. At various time points during the incubation at 37 °C with 5% CO2, triplicate plates were removed. The attached cells were then trypsinized from the surface, washed, and counted in a hemacytometer. 

Koh WG, Revzin A, Siminian A, Reeves T, Pishko M (2003) Control of mammalian cell and bacteria adhesion on substrates micropattemed with poly(ethylene glycol) hydrogels. Biomed Microdev 5 11-19... 

The behavior of the adsorption and desorption of blood proteins or adhesion and proliferation of different types of mammalian cells on pol)meric materials depends on the surface characteristics such as wettability, hydrophilicity/hydrophobicity ratio, bulk chemistry, surface charge and charge distribution, surface roughness, and rigidity. 

The adhesion of cells to surfaces is different from that of proteins and DNA. Cell adhesion and spreading are believed to depend primarily on the hydrophobicity of both cells and surfaces. Cell surface hydrophobicity is usually associated with the presence of fibrillar structures oti cell surfaces and specific cell wall proteins. Adhesion, spreading, and growth of mammalian cells are generally promoted on hydrophilic surfaces ( static 30 40°) [10], while those of bacterial... 

The attachment and growth of several types of mammalian cells on fibro-nectin-coated PDMS have been reported. All cells grew at the same rate on the PDMS irrespective of its degree of cross linking, but the compatibility of the cells on the surfaces did depend on the cell type. Similar results were obtained when micropatterns of hydrophilic regions were placed on PDMS by means of a gas plasma. These modifications of the PDMS surfaces were found to increase the adhesion of fibroblast cells. On the other hand, grafting sulfobetaine onto PDMS surfaces decreases the adsorption of blood platelets. ... 

The surface stiffness-dependent behaviour of bacteria has been less explored compared with that of mammalian cells. Using polyelectrolyte multilayer thin films composed of poly(allylamine hydrochloride) and poly(acrylic acid) as model materials, Lichter et al. found that the adhesion of Staphylococcus epidermidis is positively correlated with the stiffness of this material with a varied Young s modulus from 0.8 to 80 MPa, independent of surface roughness and charge density. Other photo-cross-linkable polyelectrolyte films made from poly(L-lysine) (PLL) and hya-luronan derivative modified with photo-reactive vinylbenzyl groups (HAVE) have also been examined. The adhesion and growth of two model bacteria, E. coli and Lactococcus lactis, were examined on the softer (non-cross-linked) and stiffer... 

Low SP, Williams KA, Canham LT, Voelcker NH (2006) Evaluation of mammalian cell adhesion on surface-modified porous silicon. Biomaterials 27 4538 Makara VA, Klyui NI, Rozhin AG, Litovchenko VG, Piryatinskii YP, Kometa OB (2003) Porous silicon photoluminescence modification by surface treatments and impregnation of carbon based nanoclusters. Phys Status Solidi A-Appl Res 197 355 Makila E, Bimbo LM, Kaasalainen M, Herranz B, Airaksinen AJ, Heinonen M, Kukk E, Hirvonen J, Santos HA, Salonen J (2012) Amine modification of thermally carbonized porous silicon with silane coupling. Langmuir 28 14045... 

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