Liquid chromatography robotic

Modern drug discovery approaches involve HTS, where, applying full automation and robotics, hundreds of molecules can be screened using several assays within a short time, and with very little amounts of compounds. In order to incorporate natural products in the modern HTS programmes, a natural product library (a collection of dereplicated natural products) needs to be built. Dereplication is the process by which one can eliminate recurrence or re-isolation of same or similar compounds from various extracts. A number of hyphenated techniques are used for dereplication, e.g. LC-PDA (liquid chromatography-photo-diode-array detector). 

Lim CK, Peters TJ (1984) Urine and faecal porphyrin profiles by reversed-phase high-performance liquid chromatography in the porphyrias. Clin Chim Acta 139 55-63 Minder El, Vuilleumier JP, Vonderschmitt DJ (1992) Prototype application of robot in the clinical laboratory enabling fully automated quantification of fecal porphyrins. Clin Chem 38 516-521... 

All possible multicomponent reactions were carried out by the combinatorial variation of between two and ten starting materials (2-CR to 10-CR) in parallel in methanol at room temperature and using a robotic dispensing system. With the aid of automated liquid chromatography and data evaluation, products were searched that are unique to a specific mixtures by comparing the retention times with the starting materials and over all other mixtures that contain the respective sub-combinations. Thus, for example, a novel product of a four-component reaction should not be contained in all three possible 3-CR mixtures. 

K. J. Halloran and H. Franze, Interaction between a robotic system and liquid chromatography, HPLC control, communication and response. In Advances in Laboratory Automation Robotics 1985 (J. R. Strimaitis and G. L. Hawk, eds.), Zymark Corp., Hopkinton, MA, 1985, p. 575. 

There are many types of automation equipment for SPE. They include semiautomated instruments, which are instruments where some intervention is required workstations, which carry out the entire SPE operation without intervention, including on-line analysis by GC and high-pressure liquid chromatography (HPLC) and customized SPE, which are robotic systems that are capable of many activities besides SPE and are custom designed for the user. 

Capillary column gas chromatography (GC)/mass spectrometry (MS) has also been used to achieve more difficult separations and to perform the structural analysis of molecules, and laboratory automation technologies, including robotics, have become a powerful trend in both analytical chemistry and small molecule synthesis. On the other hand, liquid chromatography (LC)/MS is more suitable for biomedical applications than GC/MS because of the heat sensitivity exhibited by almost all biomolecules. More recent advances in protein studies have resulted from combining various mass spectrometers with a variety of LC methods, and improvements in the sensitivity of nuclear magnetic resonance spectroscopy (NMR) now allow direct connection of this powerful methodology with LC. Finally, the online purification of biomolecules by LC has been achieved with the development of chip electrophoresis (microfluidics). 

See also-. Liquid Chromatography Overview. Quality Assurance Laboratory Information Management Systems. Sample Handling Robotics. 

V.K. Boppana, K.M. Dolce, M.J. Cyronak and J.A. Ziemniak, Simplified procedures for the determination of fenoldopam and its metabolites in human plasma by high-performance liquid chromatography with electrochemical detection comparison of manual and robotic sample preparation methods, J. Chromatogr., 1989, 487, 385-399. 

Lloyd, T.L. Gupta, S.K. Gooding, A.E. AUanti, J.R. Determination of alosetron in human plasma or serum by high-performance liquid chromatography with robotic sample preparation, J.Chromatogr.B,... 

Figure 12.2 Monitoring L-iysine concentrations in strain deveiopment. Product titers are measured quantitativeiy in the medium or cuiture supernatant, typicaiiy using liquid chromatography. To speed up strain development, a number of methods have been developed. Direct determination of product titers can be done in at-line robotic workflows involving sample preparation and analytics. Auxanographic plate...

Poly(propylene oxide)s were analysed efficiently by coupled liquid chromatography and MALDI-TOF mass spectrometry. The techniques were coupled via a robotic interface where the matrix was coaxially added to the eluate and spotted dropwise onto the MALDl target. Size exclusion chromatography and liquid chromatography at critical conditions of adsorption coupled to MALDI-TOF... 

Another unique characteristic of this technology is the potential for online structure information collection. This HTP method utilizes a high-pressure chromatography system in a parallel processing mode, i.e., multiple simultaneous column runs coupled to a robot controlled liquid handling... 

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