Lectin arrays

Analytical tools have been developed in order to identify carbohydrate structures as well as carbohydrate-binding proteins and to understand their underlying structure-function relationships of protein-carbohydrate and carbohydrate-carbohydrate interactions lectin arrays [16], glycan microarrays [17, 18], glyco-nanoparticles [19], frontal affinity chromatography [20] and carbohydrate tools for metabolic labeling [21]. 

Lectin arrays Lectin binding pmol-nmol Structural motifs Structural analysis in tissue context, living cells can be screened, no fractionation required Limited by availability and specificity of characterized lectins, quantification is challengi ng... 

Rosenfeld, R., H. Bangio, G. J. Gerwig, R. Rosenberg, R. Aloni, Y. Cohen, Y. Amor, I. Plaschkes, J. P. Kamerling, and R. B. Maya. 2007. A lectin array-based methodology for the analysis of protein glycosylation. /. Biochem. Biophys. Methods 70 415-26. 

Chemoenzymatic synthesis and lectin array characterization of a class of Ai-glycan clusters (2009) Huang et al. [51]... 

A lectin array-based methodology for the analysis of protein glyco-sylation (2007) Rosenfeld et al. [12]... 

Zheng T, Peelen D, Smith LM (2005) Lectin arrays for profiling cell surface carhohydrate expression. J Am Chem Soc 127 9982-9983... 

Kosbi Y, Nakata E, Yamane H, Hamachi I (2006) A fluorescent lectin array using supramo-lecular hydrogel for simple detection and pattern profiling for various glycoconjugates. J Am Chem Soc 128 10413-10422... 

C. F. Brewer, M. C. Miceli, and L. G. Baum, Clusters, bundles, arrays and lattices Novel mechanisms for lectin-saccharide-mediated cellular interactions, Carr. Opin. Struct. Biol., 12 (2002) 616-623. 

Glycan array allows for the definition of fine differences in specificity among related lectins giving clues about the basis for differences in their biological activities. For example comparison of a set of human galectins demonstrated that Gal-1, Gal-2, and Gal-3 all bind /V-acetyllactosamine but... 

Examination of presence of samples on array To verily that proteins, synthetic peptides, and carbohydrates are successfully printed, microarrays can be incubated with antibodies, receptors, or lectins known to react with the printed substance. The reaction is detected either by conjugating directly afluorochrome to the detector, or by a second-step staining procedure. 

The traditional methods of evaluating the interactions of glycans and glycan-binding proteins are based on isothermal titration calorimetry, surface plasmon resonance (SPR), and enzyme-linked lectin assays. Recently, glycan arrays have been developed as a... 

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