Glycan Binding Proteins

The traditional methods of evaluating the interactions of glycans and glycan-binding proteins are based on isothermal titration calorimetry, surface plasmon resonance (SPR), and enzyme-linked lectin assays. Recently, glycan arrays have been developed as a... 

Glycan Binding Proteins (GBPs) and Glycan-Protein Interactions... 

Alvarez RA, Blixt O. Identification of ligand specificities for glycan-binding proteins using glycan arrays. Methods Enzymol. 2006 415 292-310. 

Elola MT, Wolfenstein-Todel C, Troncoso MF, Vasta GR, Rabinovich GA (2007) Galectins matricellular glycan-binding proteins linking cell adhesion, migration, and survival. Cell Mol Life Sci 64 1679-1700... 

Figure 22 Photoaffinity probes for labeling glycan-binding proteins, (a) Lactose-based photoaffinity probe ° (b) N-acetyllactosamine-based photoaffinity probe. ° ...

J. Stevens, N. Razi, D.J. Stevens, J.J. Skehel, I. van Die, D.R. Burton, I.A. Wilson, R. Cummings, N. Bovin, C.-H. Wong, J.C. Paulson, Printed covalent glycan array for ligand profiling of diverse glycan binding proteins, Proc. Natl. Acad. Sci. U.S.A. 2004, 101, 17033-17038. 

H., and Paulson, J. C. (2004) Printed covalent glycan array for ligand profiling of diverse glycan binding proteins Proc Natl Acad Sci USA 101, 17033-8. 

Blixt O, Head S, Mondala T, Scanlan C, Huflejt ME, Alvarez R, Bryan MC, Fazio F, Calarese D, Stevens J, Razi N, Stevens DJ, Skehel JJ, van Die I, Burton DR, Wilson lA, Cummings R, Bovin N, Wong CH, Paulson JC. Printed covalent glycan array for ligand profiling of diverse glycan binding proteins. Proc Natl Acad Sci USA 2004 101 17033-17038. 

Animal lectin genomics resources Bacterial PolyS genes 3D structures of lectins Carbohydrate active enzymes Glycan binding proteins... 

---