K562 cancer cell line

The Gewald aminothiophene synthesis was employed in the generation of 2-amino-3-(3,4,5-trimethoxybenzoyl)-5-aryl thiophenes as derivatives of the anti-tubulin agent combretastatin A." Condensation of 2,5-dihydroxy-1,4-dithiane with 3-oxo-3-(3,4,5-trimethoxyphenyl)-propionitrile and elemental sulfur in the presence of triethylamine afforded the thiophene product in 74% yield. Further synthetic steps yielded an antitubulin agent that not only inhibited tubulin polymerization but also showed anti-proliferation activity against both L1210 and K562 cancer cell lines. 

Use of the plant in traditional Chinese medicine as an anticancer agent. Cytotoxic biflavonoids 1 and 2 against the three human cancer cell lines, HCT, NC1-H358, and K562. 

MMDC (minimum morphological deformation concentration), t No activity agaist the cancer cell line K562. 

The cytotoxicities of these compounds (Figure 8) were examined in leukemia cell line K562 cells and colon cancer cell line SW620 cells with MTS... 

Berkovic D, Berkovic K, Fleer EA, Eibl H, Unger C (1994) Inhibition of calcium-dependent protein kinase C by hexadecylphosphocholine and l-O-octadecyl-2-O-methyI-rac-glycero-3-phosphocholine do not correlate with inhibition of proliferation of HL60 and K562 cell lines. Eur J Cancer 30A 509-515... 

Cells and cell lines NIH 3T3, mouse embryonic fibroblasts K562, human chronic myeloid leukemia MDA-MB-231, human breast cancer MiaPaCa2, human pancreatic cancer n/d, not determined NSCLC, nonsmall cell lung cancer p-, phosphporylated form of a protein RB, retinoblastoma protein. 

A western blot assay on treated cancer-derived cell lines (Fig. 8.9a, b) was performed to determine the in-cell specificity of WBZ 4. The immunoblots revealed specificity toward C-Kit consistent with the selective anticancer activity on the GIST882 cell line that expresses C-Kit. Thus, the activating phosphorylation of C-Kit at sites Tyr703 and Tyr721 in ST882 cells is inhibited by WBZ 4 in a dose-sensitive manner similar to imatinib (Fig. 8.9a). By contrast, phosphorylation of Bcr-Abl at Tyr 412 (22) in K562 cells was not significantly inhibited (<15%) by WBZ 4, while densitometry revealed an imatinib-induced inhibition of 85% (Fig. 8.9b). 

M. Nakamura, K. Kirito, J. Yamanoi, T. Wainai, H. Nojiri, and M. Saito, Gang-lioside GM3 can induce megakaryocytoid differentiation of human leukemia cell line K562 cells, Cancer Res., 51 (1991) 1940-1945. 

Rao et al. [85] reported the molecular iodine-catalyzed reaction of 5,5-dimeth-yl-l,3-cyclohexanedione with aromatic aldehydes in iso-propanol affording a variety of 1,8-dioxo-octahydroxanthenes 61 (Scheme 10.43). Synthesized compounds were tested for in vitro antiproliferative properties against three caneer cell lines and 9-(2-hydroxyphertyl)-3,3,6,6-tetramethyl-3,4,5,6,7,9-hexahydro-l/f-xanthene-l,8(2/f)-dione possessing a 2-hydroxy phenyl group at C-9 positioa Antiproliferative properties of these eompoimds were also evaluated in vitro against a number of cancer eell lines, for example, human chronic myeloid leukemia cells (K562), human colon carcinoma cells (Colo-205), and human neuroblastoma cells (IMR32). 

Cranberries (Vaccinium macrocarpon) are potentially an excellent dietary source of phenolic confounds such as flavonoids, anthocyanins and caffeic acid derivatives which are potent antioxidants. Studies also link a lowered incidence of breast cancer to cranberry juice consumption. In this study, cranberry fruits were fractionated by several methods and tested for radical-scavenging activity in an effort to begin establishing a link between chemical composition and antioxidant activity. The strongest activity was observed in flavonoid-rich extracts. Cytotoxicity assays in several tumor cell lines showed some specificity for HT-29 tumor cells and K562 cells from a methanolic cranberry extract containing several phenolic compounds. 

Ray et al. studied the different cytotoxic effects for a number compormds, including various tributyltin halobenzoates on human leukemic K562 cells. They also studied the role of extraceUrrlar calcium ion influx and ROS formation in inducing apotosis in this cell line. Human breast cancer MCF-7 cells were also employed in the studies. The R562 has a low membrane cholesterol content, while the MCF-7... 

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