Intracellular sorting

Vijayasaradhi, S., Xu, Y., Bouchard, B., and Houghton, A. (1995). Intracellular sorting and targeting of melanosomal membrane proteins identification of signals for sorting of the human brown locus protein, gp75. J. Cell Biol. 130, 807-820. 

Serum Tfn belongs to a class of metal-binding glycoproteins of approximately SOkDa in size and is often used as an essential tool for studying cellular uptake and the subsequent intracellular sorting and recycling. 

Because the intracellular pH at least partially regulates the intracellular sorting of endocytosed ligands (61), a block of acidification leads to a change of intracellular distribution and diminished uptake (82). 

Macromolecules, such as proteins and peptides, are taken up by polarized epithelial cells by random pinocytosis, i.e., sampling of luminal fluid containing such molecules into apical early endosomes (AEE) as shown for MDCK cells in vitro (Leung et al. 2000). Further intracellular sorting is depicted in general terms in Fig. 3.3. Recycling to the apical surface or transcytosis is then mediated... 

Fig. 3.3 Intracellular sorting in epithelial cells after clathrin-mediated endocytosis. AEE apical early endosome ARE apical recycling endosome CE common endosome ...

Fig. 3.4 Intracellular sorting by caveolae.CV caveosome ER endoplasmatic reticulum GC Golgi complex...

Sandvig K, Garred O, van Helvoort A, van Meet G, Van Deurs B, Importance of glycolipid synthesis for butyric acid-induced sensitization to Shiga toxin and intracellular sorting of toxin in A431 cells, Mol Biol Cell, 1996 7 1391 -1404. 

D Urso D, Muller HW. Ins and outs of peripheral myehn protein-22 mapping transmembrane topology and intracellular sorting. J Neurosci Res 1997 49 551-62. 

Moisenovich, M., Tonevitsky, A., Agapov, I., Niwa, H., Schewe, H. and Bereiter-Hahn, J. (2002) Differences in endocytosis and intracellular sorting of ricin and viscumin in 3T3 cells. Eur J Cell Biol, 81, 529-538. 

Figure 8.4. Intracellular sorting pathways of RME. The initial binding and uptake steps [including receptor clustering in eoated or noncoated pits, internalization of the receptor-ligand complex into coated vesicles (noneoated in the ease of potoc3d osis), and fusion of vesicles to form endosomes] are common to all pathways. After entry into acidic endosomes, ligand and receptors are sorted and trafficked independently, whieh may result in degradation, recycling or transcytosis of either molecule (seetext). L, ligand R, receptor lysosomes are depicted as shaded circles. (Adaptedfrom Ref 10.)...

Webster, P. and Fish, W. R. (1989) Endocytosis by African trypanosomes. II. Occurrence in different life-cycle stages and intracellular sorting. Eur. J. Cell Biol. 49 303-310. 

Omura, T. and A. Ito (1991). Biosynthesis and intracellular sorting of mitochondrial forms of cytochrome P450. Meth. Enzymol. 206, 75-81. 

Glycoproteins are essential to many basic cellular and disease processes including molecular/cellular recognition, intracellular sorting, cell growth, fertilization, immune defense, inflanunation, tumor metastasis, viral replication and bacterial/parasite infection. For example, carbohydrates of glycoproteins at the manunalian cell surface have two major functions. Inside the cell, they help proteins fold and assemble correctly in the... 

Lisanti, M., Le Bivic, A., Sargiacomo, M., and Rodriguez-Boulan, E. (1989) Steady state distribution and biogenesis of endogenous MDCK glycoproteins Evidence for intracellular sorting and polarized cell surface delivery. /. Cell Biol. 109, 2117-2128. 

Krieger, M, Reddy, P. Kozarsky, K, Kingsley, D. Hobbie, L. Penman, M. Analysis of the synthesis, intracellular sorting, and function of glycoproteins using a mammalian cell mutant with reversible glycosylation defects. Methods Cell Biol. 1989, 32, 57-84. 

Several precedents exist for the intracellular sorting of lipids to different membranes according to fatty acid chain length [51, 52],... 

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