Glycoproteins using

The Biosynthesis of O-Linked Glycoproteins Uses Nucleotide Sugars... 

L. (2005) Classification of substrates and inhibitors of P-glycoprotein using unsupervised machine learning approach. Journal of Chemical Information and Computer Sciences, 45, 750-757. 

Loo, T.W. and Clarke, D.M. (1999) Determining the structure and mechanism of the human multidrug resistance P-glycoprotein using cysteine-scanning mutagenesis and thiol-modification techniques. Biochimica et Biophysica Acta, 1461, 315-325. 

Singh, S. Scigelova, M. Crout, D. H. G., Glycosidase-catalysed synthesis of oligosaccharides A two-step synthesis of the core trisaccharide of N-hnked glycoproteins using the beta-N-acetylhexosaminidase and the beta-mannosidase from... 

Roffman, E., Meromsky, L., Ben-Hur, H., Bayer, E. A., and Wilchek, M. (1986) Selective labeling of functional groups on membrane proteins or glycoproteins using reactive biotin derivatives and 1-streptavidin. Biochem. Biophys. Res. Comm. 136, 80-85. 

MER Rosas, A Shibukawa, Y Yoshikawa, Y Kuroda, T Nakagawa. Binding study of semotiadil and bevosemotiadyl with aracid glycoprotein using high-performance frontal analysis. Anal Biochem 274 27-33, 1999. 

These are discussed elsewhere but include a recombinant vaccinia virus that expresses rabies virus glycoproteins used for oral vaccination. 

A how-to manual for the isolation and characterization of the oligosaccharide moieties of glycoproteins, using the whole range of modern techniques. Available as part of the IRL Press Practical Approach Series on CD-ROM, from Oxford University Press (www.oup-usa.org/acadsci/pasbooks.html). 

Huwyler, J., et al. 2002. By-passing of P-glycoprotein using immunoliposomes. J Drug Target 10 73. 

L. M. Likhosherstov, O. S. Novikova, V. A. Derevitskaya, and N. K. Kochetkov, New method for cleavage of W-linked carbohydrate chains of glycoproteins using alkaline lithium borohyd-ride, Dokl. Akad. Nauk SSSR, 274 (1984) 222-225. 

N. Kawasaki, M. Ohta, S. Hyuga, O. Hashimoto, and T. Hayakawa, Analysis of carbohydrate heterogeneity in a glycoprotein using liquid chromatography/mass spectrometry and liquid chromatography with tandem mass spectrometry, Anal. Biochem., 269 (1999) 297-303. 

Hancock, W. S. Apffel, A. Chakel, J. Souders, C. M Timkulu, T. Pungor, E. Guzzetta, A. W. 1996. Reversed-phase peptide mapping of glycoproteins using liquid chromatography/electrospray ionization-mass spectrometry. Methods Enzymol., 271,4QA-421. 

Mazsaroff, I. Yu, W. Kelley, B. D. Vath, J. E. 1997. Quantitative comparison of global carbohydrate structures of glycoproteins using LC-MS and in-source fragmentation. Anal Chem., 69,2517-2524. 

M. Chaplin, A rapid and sensitive method for the analysis of carbohydrate compounds in glycoproteins using gas-liquid chromatography, Anal. Biochem., 723(2) 336-341 (1985). 

Loo TW, Clarke DM (2001) Determining the dimensions of the drug-binding domain of human P-glycoprotein using thiol cross-linking compounds as molecular rulers. Biol Chem 276 36877-36880... 

H. Zhang, X.-J. Li, D. B. Martin, and R. Aerbersold, Identification and quantification of W-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry, Nat. Biotechnol., 21 (2003) 660-666. 

C. Sihlbom, I. D. Hard, M. E. Lidell, T. Noll, G. C. Hansson, and M. Backstrom, Localization of O-glycans in MUC1 glycoproteins using electron-capture dissociation fragmentation mass spectrometry, Glycobiology, 19 (2009) 375-381. 

Zhang H, et al. Identification and quantification of N-linked 45. glycoproteins using hydrazide chemistry, stable isotope labeling... 

Weitzhandler, M. Hardy, M. Co, M.S. and Avdalovic, N. J. Pharm. Sci. 1994, in press. Townsend, R. Quantitative Monosaccharide Arudysis of Glycoproteins Using HPLC, 1994 in Chromatography in Biotechnology editors Horvath, C. and Ettre, L. S. ACS, Washington DC, ACS Symosium Series 529. 

---