Hippocampal slice intracellular recording

Fig. 27. Intracellular recordings from a granule cell in the hippocampal slice to show the response to the iontophoretic application of GABA. (A) Voltage record made on moving film to show the way in which an intracellular injection of a depolarizing ramp of current is used to test the cell s excitability. Every alternate second a hyperpolarizing current pulse is used to measure the input resistance. As shown more clearly in the single shots in (B) and (C), the application of GABA inhibited the spike discharge evoked by the ramp, caused a substantial decrease in the input resistance, and produced a small depolarization with respect to resting membrane potential [dotted line in (B)]. (From Assaf et ai, 1981.)...

Dingledine, R., Dodd, J., and Kelly, J. S., 1977a, Intracellular recording from pyramidal neurones in the in vivo transverse hippocampal slice,/. Physiol. 269 13-15P. 

Aniracetam reversibly potentiated the ionotropic quisqualate responses recorded intracellularly from the pyramidal cells in the CAl region of rat hippocampal slices (ITO et al. 1990). The excitatory postsyn-aptic potentials in Schaffer collateral-commissural-CAl synapses was also potentiated by aniracetam. 

The 5-HT1A receptors are coupled to potassium and calcium channels. Intracellular current-clamp recordings in slices containing the DR established that the 5-HT-mediated inhibition involved an increase in potassium conductance, which exhibits inward rectification (18,58). This induced a membrane hyperpolarization leading to a decrease in action potential frequency. Similar responses to 5-HT1A receptor activation have been reported in other neuronal types, such as hippocampal pyramidal cells (16,59) or 5-HT neurons of the caudal raphe nuclei (60). 

Schwartzkroin, P. a., 1975, Characteristics of CAl neurones recorded intracellularly in the hippocampal in vitro slice preparation. Brain Res. 85 423-436. 

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