Brain slices intracellular recording from neurons

INTRACELLULAR RECORDING FROM NEURONS IN BRAIN SLICES IN VITRO... 

A number of studies in fact show clear Di effects. Intracellular recording from striatal neurons in rat brain slices show a cAMP-mediated Di-dependent (blocked by SCH 23390) suppression of a voltage-dependent sodium current which make the cell less responsive. 

Figure 1.9 Comparison of the effects of an endogenously released and exogenously applied neurotransmitter on neuronal activity (identity of action). Recordings are made either of neuronal firing (extracellularly, A) or of membrane potential (intracellularly, B). The proposed transmitter is applied by iontophoresis, although in a brain slice preparation it can be added to the bathing medium. In this instance the applied neurotransmitter produces an inhibition, like that of nerve stimulation, as monitored by both recordings and both are affected similarly by the antagonist. The applied neurotransmitter thus behaves like and is probably identical to that released from the nerve...

The immediate and reversible actions of dopamine are a combination of modulations of individual ion channels. The major ion channels expressed in spiny projection neurons are summarized in Table 2. Current understanding of the properties of these channels is mostly based on whole cell recordings from isolated cells, which have been recently reviewed by Nichola et al. (2000). The role of these channels in whole cell behavior has been studied using intracellular recordings in brain slices or anaesthetized animals. Many of the important cellular properties of spiny projection neurons can be accounted for in terms of ion channel activations occurring at different membrane potentials. 

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