Integrins laminin

Zhang S, Fulton AM. Modulation of integrin-laminin receptor function on mammary tumor cells by prostaglandin E2 receptor antagonism. Cancer Lett 1994 85 233-238. 

L.F. (1995) Expression and in vitro funetion of beta(l) -integrin laminin receptors in the developing avian ciliary ganglion. 7. Neurosci. 15 5275-5285. 

GlukhovaM, KotelianskyVE, FondacciC, Marotte F, RappaportL (1993) Laminin variants and integrin laminin receptors in developing and adult human smooth muscle cells. Dev Biol 157 437-447... 

Figure 48-4. Schematic representation of a cell interacting through various integrin receptors with collagen, fibronectin, and laminin present in the ECM. (Specific subunits are not indicated.) (Redrawn after Yamada KM Adhesive recognition sequences. J Biol Chem 1991 266 12809.)...

Integrins themselves are found on nearly all cells and mediate several physiological responses, such as cell-cell and cell-matrix interactions. Three families of integrins, each family with a common beta subunit in combination with distinct alpha subunits, have been recognized. The beta 1 family, also called very late lymphocyte-activation antigen or VLA, has receptors mediating extracellular matrix interactions with molecules such as collagen, laminin, and fibronectin. Naturally, platelets contain many of the receptors of the beta 1 family. 

Thus, while glycoprotein Ia/IIa (a2Pj) binds to collagen, glycoprotein Ic/IIa (a ) binds to fibronectin (5). The other integrin belonging to the beta 1 family that is involved in platelet adhesion is ct6P, which binds laminin. 

Cells are sometimes cultured in serum-free medium. In this condition, the surface should carry substituents of serum proteins that can directly interact with cells. SAMs of alkanethiols with bioactive ligands have been used to control interactions between the material surface and cells [80-83]. Several bioactive ligands have been tested, including RGD [80], PHSRN [81], and laminin-derived peptides [82, 83]. These ligands were expected to directly interact with cell surface integrins. 

Another 3-D cornea model, comprising rabbit primary cultures of epithelial and stromal cells as well as mouse immortalized endothelial cells, was described in 1994 by Zieske and coworkers [70], They showed the influence of endothelial cells on the formation of a tightly packed, multilayered epithelium as well as the expression of laminin, type VII collagen, a6 integrin, keratin K3, and a-enolase. Furthermore, their findings suggested that the formation of an in vivo-like epithelium requires the cultivation of the 3-D corneal construct under AIC conditions. By contrast, LCC methods of cultivating corneal equivalents in the absence of endothelial cells failed to promote the expression of differentiation markers and basement membrane components. 

YIGSR, IKVAV Laminin Integrins aOpi, a pi Neurons... 

Finally, the matrix must be attached to the cell process and the canalicular wall in order for the drag force to be transmitted to the membrane and its underlying intracellular actin cytoskeleton. If such linker molecules are present, drag forces exerted on the matrix fibers will produce a tensile stress on these linker molecules that, in turn, will produce radial (hoop) strain in the intracellular actin cytoskeleton as schematically shown in Figure 6. Possible candidates for these attachment molecules are CD44, laminin, and various integrins. [64, 149],... 

V. Carloni, R. G. Romanelli, M. Pinzani, G. Laffi, and P. Gentilini, Expression and function of integrin receptors for collagen and laminin in cultured human hepatic stellate cells, Gastroenterology 110 1127-1136 (1996). 

Cell attachment to the glomerular basement membrane is mediated by laminin, entactin, and fibronectin, at least partly through integrin receptors on the cell surface of both endothelial and visceral epithelial cells. 

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