Integration by homologous recombination

Expression of the B.t. protein in strains with the B.t. gene integrated by homologous recombination was confirmed by Western blot analysis. The strains tested produced a 134 kD protein which retained toxicity to susceptible insects (ffanduca sexta). 

Rossolini, G.M., Riccio, M.L., Gallo, E., and Galeotti, C.L. (1992) Kluyveromyces lactis rDNA as a target for multiple integration by homologous recombination. Gene, 119, 75-81. 

Eukaryotic Yeast plasmid or integration into host chromosome by homologous recombination Transient or permanent Amino acid requirement in autotrophic strain heavy metal induction of resistance gene Yeast ori sequence constitutive or inducible promoter transcription terminator... 

Lida, S., Terada, R. 2004. A tale of two integrations, transgene and T-DNA gene targeting by homologous recombination in rice. Curr Opin Biotechnol 15 132-138. 

Yip Vectors The Ypl integrative vectors do not replicate autonomously, but integrate into the genome at low frequencies by homologous recombination. Integration of circular plasmid DNA by homologous recombination leads to a copy of the vector sequence flanked by two direct copies of the yeast sequence. 

Poncet, S., C. Bernard, E. Dervyn, J. Cayley, A. Klier, and G. Rapoport. 1997. Improvement of Bacillus sphaericus toxicity against dipteran larvae by integration, via homologous recombination, of the CryllA toxin gene from Bacillus thuringiensis subsp. israelensis. Appl. Environ. Microbiol. 63 4413-4420. 

DeVries, J. and Wackernagel, W. (2002). Integration of foreign DNA during natural transformation of Acinetobacter sp. by homology-facilitated illegitimate recombination , Proc. Natl. Acad. Sci. USA, 99, 2094-2099. 

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