Integration by homologous

Expression of the B.t. protein in strains with the B.t. gene integrated by homologous recombination was confirmed by Western blot analysis. The strains tested produced a 134 kD protein which retained toxicity to susceptible insects (ffanduca sexta). 

Rossolini, G.M., Riccio, M.L., Gallo, E., and Galeotti, C.L. (1992) Kluyveromyces lactis rDNA as a target for multiple integration by homologous recombination. Gene, 119, 75-81. 

DeVries, J. and Wackernagel, W. (2002). Integration of foreign DNA during natural transformation of Acinetobacter sp. by homology-facilitated illegitimate recombination , Proc. Natl. Acad. Sci. USA, 99, 2094-2099. 

Eukaryotic Yeast plasmid or integration into host chromosome by homologous recombination Transient or permanent Amino acid requirement in autotrophic strain heavy metal induction of resistance gene Yeast ori sequence constitutive or inducible promoter transcription terminator... 

Lida, S., Terada, R. 2004. A tale of two integrations, transgene and T-DNA gene targeting by homologous recombination in rice. Curr Opin Biotechnol 15 132-138. 

The electron transfer, as observed in vitro between these two proteins, is believed to occur through the formation of a speciflc complex between the two proteins, being the interaction mainly electrostatic in nature. The nature and properties of the protein-protein complex (stoichiometry, interaction sites, association constants) were probed. Integration and correlation of the experimental results obtained from magnetic resonance studies on protein-protein titrations with the available structural and biochemical data is presented. A structural model for a hypothetical ternary complex, formed between one molecule of flavodoxin and two molecules of cytochrome C3, is proposed using the available X-ray structures of the isolated proteins and, when required, model structures predicted by homology modeling. 

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