Insulin using rabbits

Normal Rabbits. Six male, white rabbits (2.5 - 3.0 kg) were housed individually. Animals were fasted overnight for 16 hours (with access to water) prior to each experiment to reduce the gastrointestinal content and absorption variability. After treatment with either a control dose or experimental insulin in poly(acrylic acid) resin dose, a one week washout period was required before the next experiment. The protocol called for blood samples to be taken from an indwelling ear catheter at -1, -.5, -.25, +.5, +1, +1.5, +2, +3, +4, +5 and +6 hours. Serum glucose levels were determined by an oxidase colorimetric method using the Sigma 510 Glucose Kit. 

Hosny, E.A., et al. 1997. Hypoglycemic effect of oral insulin in diabetic rabbits using pH-dependent coated capsules containing sodium salicylate without and with sodium cholate. Pharm Acta Helv 72 203. 

Lee, S. 2004. Noninvasive ultrasonic transdermal insulin delivery in rabbits using the light-weight cymbal array, Diabetes Technol Ther 6 808. 

Ando, T., et al. 1998. Nasal insulin delivery in rabbits using soybean-derived sterylglucoside and sterol mixtures as novel enhancers in suspension dosage forms. Biol Pharm Bull 21 862. 

Morimoto, K., et al. 1982. Effective vaginal absorption of insulin in diabetic rats and rabbits using polyacrylic acid aqueous gel bases. Int J Pharm 12 107. 

Polyclonal antibodies have been prepared against partially purified rabbit mammary gland receptors [42-44], and have been used to demonstrate that the receptors really are involved in mediating the biological actions of prolactin. Anti-receptor antibodies that blocked binding of labelled prolactin were able to inhibit prolactin-stimulated casein synthesis and amino acid transport in rabbit mammary gland explants in vitro [43,45] (Fig. 4). They had no effect on the actions of insulin... 

Carcinogenicity used the 12-month repeat dose study instead of a standard 2-year bioassay Other cardiovascular study in dogs drug interaction study with NPH insulin immunogenicity in rabbits In vitro immunology, and Athymic nude mice proof of concept studies... 

Groups of 4-5 mixed breed rabbits of either sex weighing 3.0-4.5 kg are used. For insulin evaluation, food is withheld overnight. For evaluation of sulfonylureas and other blood glucose lowering agents the animals... 

Since the purpose of this report is to illustrate the usefulness of Z-gel as a reagent for the separation of antigens from antigen-antibody complexes, the methods used for the purification of CEA and AFP will not be described. Bovine insulin was purchased from Schwarz-Mann, and anti-bovine insulin serum was purchased from Miles Laboratories. Antisera to CEA and AFP were prepared in goats and rabbits, respectively. Standard solutions of nonradioactive CEA (125 ng/ml) and AFP (180 ng/ml) are prepared in 50 mM borate buffer (pH 8.4) containing 10% (v/v) group A human plasma for CEA or 0.25% human albumin for AFP and 0.01% thimerosal. The bovine insulin standard (100 /u.IU/ml) is prepared in 40 mM phosphate buffer, pH 7.4, containing 0.15 M NaCl, 0.5% bovine serum albumin (BSA), and 0.01% thimerosal (phosphate buffer 1). 

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