Injection system capillary electrophoresis

A. Manz, D.J. Harrison, E.M.J. Verpoorte, J.C. Fettinger, A. Paulus, H. Ludi and H.M. Widmer, Planar chips technology for miniaturization and integration of separation techniques into monitoring systems Capillary electrophoresis on a chip, J. Chromatogr. A, 593 (1992) 253-258. D.J. Harrison, A. Manz, Z.H. Gan, H. Ludi and H.M. Widmer, Capillary electrophoresis and sample injection systems integrated on a planar glass chip, Anal. Chem., 64 (1992) 1926-1932. 

Chen, H.-W., Fang, Z.-L., Combination of flow injection with capillary electrophoresis. Part 3. Online sorption column preconcentration capillary electrophoresis system. Anal. Chim. Acta 1997, 355(2-3), 135-143. 

P Kuban, M. Berg, C. Garcia and B. Karlberg, On-hne flow sample stacking in a flow injection analysis-capillary electrophoresis system 2000-fold enhancement of detection sensitivity for priority phenol pollutants, J. Chromatogr. A, 912, 163-170, 2001. 

Huang XJ, Wang SL, Fang ZL (2002) Combination of flow injection with capillary electrophoresis 8, Miniaturized capillary electrophoresis system with flow injection sample... 

Kuban P. and Karlberg B., On-line monitoring of kraft pulping liquors with a valveless flow injection-capillary electrophoresis system, Anal. Chim. Acta, 404, 19, 2000. 

Fan et al. [106] developed a high performance capillary electrophoresis method for the analysis of primaquine and its trifluoroacetyl derivative. The method is based on the mode of capillary-zone electrophoresis in the Bio-Rad HPE-100 capillary electrophoresis system effects of some factors in the electrophoretic conditions on the separation of primaquine and trifluoroacetyl primaquine were studied. Methyl ephedrine was used as the internal standard and the detection was carried out at 210 nm. A linear relationship was obtained between the ratio of peak area of sample and internal standard and corresponding concentration of sample. The relative standard deviations of migration time and the ratio of peak area of within-day and between-day for replicate injections were <0.6% and 5.0%, respectively. 

Capillary electrophoresis narrow-bore fused-silica capillary tube injection system detector recorder orVDU. 

Some coupled systems allow measurement of the main N and P forms (nitrate, ammonia and orthophosphates) [22,27,29], among which is a system based on membrane technology in combination with semi-micro continuous-flow analysis (pCFA) with classical colorimetry. With the same principle (classical colorimetry), another system [30] proposes the measurement of phosphate, iron and sulphate by flow-injection analysis (FIA). These systems are derived from laboratory procedures, as in a recent work [31] where capillary electrophoresis (CE) was used for the separation of inorganic and organic ions from waters in a pulp and paper process. Chloride, thiosulphate, sulphate, oxalate,... 

L. Arce, A. Rios and M. Valcarcel, Determination of anti-carcinogenic polyphenols present in green tea using capillary electrophoresis coupled to a flow injection system. J. Chromatogr.A 823 (1998) 113-120. 

Figure 9.1 Schematic representation of a basic capillary electrophoresis system. The main components include a capillary (commonly contained within a housing that allows for temperature control), a power supply, and a detector. Automation is achieved through the use of computer-controlled setting of solutions and samples, displacement forces (to replace capillary contents and for hydrodynamic injection), and automatic data collection. (Courtesy of Agilent Technologies.)...

Harrison, D. J., Manz, A., Pan, Z., Liidi, H., and Widmer, H. M. (1992). Capillary electrophoresis and sample injection systems integrated on a planar glass chip. Anal. Chem. 64, 1926-1932. 

Capillary electrophoresis has also been used to examine the enantiomeric purity of brinzolamide. The system consisted of a Polymicro Technologies fused silica capillary column (50 cm x 50 pm), UV detection at 255 nm, pH 2.4 buffer (28 mM heptakis-(2,6-di-o-methyl)-P-cyclodextrinand 30 mM TRIS-(hydroxylmethylaminomethane),adjusted to pH with 85% H3PO4) and an applied voltage of 20 kV. Injection of a 200 pg/mL sample was accomplished with 50 mm gravity for 10 seconds. 

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