Peptide nucleic acid probe

Hongmanee P., Stender H., Rasmussen O.F. Evaluation of a fluorescence in situ hybridization assay for differentiation between tuberculous and nontuberculous Mycobacterium species in smears of Low-enstein-Jensen and mycobacteria growth indicator tube cultures using peptide nucleic acid probes. J. Clin. Microbiol. 2001 39 1032-1035. 

Raymond FR, Ho H-A, Peytavi R, Bissonnette L, Boissinot M, Picard FJ, Leclerc M, Bergeron MG (2005) Detection of target DNA using fluorescent cationic polymer and peptide nucleic acid probes on solid support. BMC Biotechnol 5 10... 

Gaylord BS, Massie MR, Feinstein SC, Bazan GC (2005) SNP detection using peptide nucleic acid probes and conjugated polymers applications in neurodegenerative disease identification. Proc Natl Acad Sci USA 102 34—39... 

Gaylord BS, Heeger AJ, Bazan GC (2002) DNA detection using water-soluble conjugated polymers and peptide nucleic acid probes. Proc Natl Acad Sci USA 99 10954—10957... 

An array of oligonucleotide which is composed of 20 80-mer oligos, or peptide nucleic acid probes, is synthesized either in situ (i.e., on-chip) or using conventional synthesis followed by on-chip immobilization. The resultant DNA array is then exposed to the labeled sample of DNA, hybridized, and the identity/abundance of complementary sequences determined. This method was developed at Affymetrix, Inc. and called DNA chips. Today, oligonucleotide-based chips are manufactured by many companies using alternative in situ synthesis or depositioning technologies. 

A novel technique that combines the unique performance characteristics of peptide nucleic acid probes (PNA) with fluorescence in situ hybridization (FISH) for identification of S. aureus directly from positive blood cultures without cultivation and biotyping has been described (Kempf et al. 2000) and has recently been applied to the rapid and specific identification of bacteria including S. aureus (Oliveira et al. 2003). 

Label-free and label based electrochemical detection of hybridization by using methylene blue and peptide nucleic acid probes at chitosan modified carbon paste electrodes. Electroanalysis 14, 1685-1690. 

Kerman, K., Ozkan, D., Kara, P., Erdem, A., Meric, B., Nielsen, P. E., Ozsoz, M. (2003). Label-free bioelectronic detection of point mutation by using peptide nucleic acid probes. Electroanalysis 15, 667-670. 

FIT probes peptide nucleic acid probes with a fluorescent base surrogate enable real-time DNA quantification and single nucleotide polymorphism discovery. Anal Biochem., 375, 318-330. 

Stender, H., Kurtzman, C., Hyldig-Nielsen, J. J., Sorensen, D., Broomer, A., Oliveira, K., et al. (2001). Identification of Dekkera bruxeUensis (Brettanomyces) from wine by fluorescence in situ hybridization using peptide nucleic acid probes. Applied and Environmental Microbiology, 67, 938-941. 

Ahneida, C., Azevedo, N. R, Fernandes, R. M., Keevil, C. W., Vieira, M. J. (2010). Fluorescence in situ hybridization method using a peptide nucleic acid probe for identification of Salmonella spp. in a broad spectrum of samples. Applied and Environmental Microbiology, 76(13), 4476-4485. http //dx.doi.org/10.1128/AEM.01678-09 PMCID PMC2897454. 

Nilsson, K. P. R. Inganas, O. Chip and solution detection of DNA hybridization using a luminescent zwitterionic polythiophene derivative. Nat. Mater. 2003, 6, 419 24. Raymond, F. R. Ho, H.-A. Peytavi, R. Bissonnette, L. Boissinot, M. Picard, F. J. Leclerc, M. Bergeron, M. G. Detection of target DNA using fluorescent cationic polymer and peptide nucleic acid probes on solid support. BMC Biotechnol. 2005,10, 1-5. 

Wang, J., Palecek, E., Nielsen, P.E. et al. (1996) Peptide nucleic acid probes for sequence-specific DNA biosensors. Biochemistry, 7863 (10), 7667-7670. 

Ribeiro Pinto de Oliveira Azevedo, N. F Macieira Cerqueira, L. I. Torres Faria, N. R. Lopes da Costa Vieira, M. J. Methods and peptide nucleic acid probes for specific detection of Helicobacter pylori and its response to clarithromycin. PCT Int. Appl. WO 2011030319,2011. 

C. W. Peptide nucleic acid probe, kit and method for detection and/or quantification of Salmonella spp. and applications thereof. PCX Int. Appl. WO... 

Chandler, D.P. et al.. Affinity capture and recovery of DNA at femtomolar concentrations with peptide nucleic acid probes. Analytical Biochemistry, 2000 283(2) 241-249. 

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