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Immunoglobulin immunosorbent assay

In each of the assays of potency the amount of the immunoglobulin and the amount of a corresponding standard preparation that are required to neutralize the infectivity or other biological activity of a defined amount of virus or to neutralize a defined amount of a bacterial toxin are determined. The two determined amounts and the assigned unitage of the standard preparation are then used to calculate the potency of the immunoglobulin in International Units (lU). ELISA, enzyme-linked immunosorbent assay. [Pg.319]

Antiphospholipid antibodies include lupus anticoagulants (LAs) and anticardi-olipin (aCL) antibodies. Lupus anticoagulants are immunoglobulins that are characterized by their ability to inhibit phospholipid-dependent coagulation assays. In contrast, aCL antibodies are measured in an enzyme-linked immunosorbent assay... [Pg.155]

King, D.P. et al., The use of monoclonal antibodies specific for seal immunoglobulins in an enzyme-linked immunosorbent assay to detect canine distemper virus-specific immunoglobulin in seal plasma samples, J. Immuno. Methods, 160, 163, 1993. [Pg.416]

Niewola et al. [183, 185] have described a rapid, convenient and accurate method, based upon an enzyme-based immunosorbent assay (ELISA) for the determination of Paraquat residues in soil. Polystyrene plates, coated with paraquat-keyhole limpet haemocyanin (KLH) conjugate, are incubated with the test samples and a known amount of monoclonal antibody. Residual antibody that has not reacted with free Paraquat in the sample combines with paraquat-KLH on the plate. The determination of the fixed antibody is achieved by the addition of peroxidase labelled rabbit antimouse immunoglobulin G followed by reaction with a chromogenic substrate. The enzyme activity of the solid phase is determined from the absorbance measurements, which are inversely proportional to the concentration of Paraquat. The method shows high specificity and correlates well with the traditional ion exchange-spectrophotometric method for the determination of Paraquat [178]. [Pg.258]

Engvall E, Jonsson K, Perlmann P. 1971. Enzyme-linked immunosorbent assay. II. Quantitative assay of protein antigen, immunoglobulin G, by means of enzyme-labelled antigen and antibody-coated tubes. Biochim Biophys Acta 251 427-434. [Pg.217]

Engvall, E. and Perlman, P. (1971) Enzyme-linked immunosorbant assay (ELISA) quantitative assay of Immunoglobulin G. Immunocytochemistry 8, 871-879. [Pg.239]

K. Sato, M. Tokeshi, T. Odake, H. Kimura, T. Ooi, N. M. and T. Kitamori Integration of an Immunosorbent Assay System Analysis of Secretory Human Immunoglobulin a on Polystyrene Beads in a Microchip. Anal. Chem. 72, 1144 (2000). [Pg.218]

SpA, Staphylococcal protein A PpL, protein L from Peptostreptococcus Magnus IgG, immunoglobulin G Gla, y-carboxyglutamic acid SPR, surface plasmon resonance ELISA, enzyme-linked immunosorbent assay FITC, fluorescein isothiocyanate NMR, nuclear magnetic resonance... [Pg.46]

Sato, K., Tokeshi, M., Odake, T., Kimura, H., Ooi, T., Nakao, M., Kitamori, T., Integration of an immunosorbent assay system Analysis of secretory human immunoglobulin A on polystyrene beads in a microchip. Anal. Chem. 2000, 72, 1144-1147. [Pg.454]

E5. Engvall, E., and Perlmann, P., Enzyme linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. Immunochemistry 8, 871-874 (1971). [Pg.105]

ELISA enzyme-linked immunosorbent assay IgA immunoglobulin A... [Pg.44]

Prince HE. Evaluation of the INOVA diagnostics enzyme-linked immunosorbent assay kits for measuring serum immunoglobulin G (IgG) and IgA to deamidated gliadin peptides. Clin Vaccine Immunol 2006 13 150-151. [Pg.62]

Godfrey, M. A., Kwasowski, P., Clift, R., and Marks, V. (1992). A sensitive enzyme-linked immunosorbent assay (ELISA) for the detection of staphylococcal protein A (SpA) present as a trace contaminant of murine immunoglobulins purified on immobilized protein A. J. Immunol. Methods 149, 21-27. [Pg.628]

Biagini, R.E., Sammons, D.L., Smith, J.P., MacKenzie, B.A., Striley, C.A.F., Semenova, V., Steward-Clark, E., Stamey, K., Freedman, A.E., Quinn, C.P., Snawder, J.E. (2004). Comparison of a multiplexed fluorescent covalent microsphere immunoassay and an enzyme-linked immunosorbent assay for measurement of human immunoglobulin G antibodies to anthrax toxins. Clin. Diag. Lab. Immunol. 11 50-5. [Pg.454]

Quinn, C.P., Semenova, V.A., Elie, C.M., Romero-Steiner, S., Greene, C., Li, H., Stamey, K. (2002). Specific, sensitive, and quantitative enzyme-linked immunosorbent assay for human immunoglobulin G antibodies to anthrax toxin protective antigen. Emerg. Infect. Dis. 8 1103-10. [Pg.458]

The titer of an antibody describes the immunoglobulin concentration in serum and is a measure of the highest dilution that will still give a visible antibody-antigen precipitation. Higher antibody titers are usually obtained after repeated antigen boosts. Antibody titers can be determined by double-diffusion assays in gels, enzyme-linked immunosorbent assays... [Pg.7]

Engrail, E. Perlman, P. Enzyme-linkend immunosorbent assay (ELISA) quantitative assay of immunoglobulin. G. Immunochemistry 1971, 8, 871-874. [Pg.2061]

IgA, Immunoglobulin A ELISA, enzyme-linked immunosorbent assay. [Pg.1861]

Talarmin A, Labeau B, Lelarge J, Sarthou JL (1998) Immunoglobulin A-specific capture enzyme-linked immunosorbent assay for diagnosis of dengue fever. J Clin Microbiol 36(5) 1189-1192... [Pg.294]

See other pages where Immunoglobulin immunosorbent assay is mentioned: [Pg.240]    [Pg.241]    [Pg.473]    [Pg.68]    [Pg.562]    [Pg.15]    [Pg.7]    [Pg.43]    [Pg.163]    [Pg.305]    [Pg.262]    [Pg.424]    [Pg.230]    [Pg.445]    [Pg.388]    [Pg.46]    [Pg.2689]    [Pg.121]    [Pg.286]    [Pg.294]   
See also in sourсe #XX -- [ Pg.2 , Pg.325 , Pg.326 ]



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