Fluorescence multiplexing

Duponchel C, Di Rocco C, Cicardi M, Tosi M. Rapid detection by fluorescent multiplex PCR of exon deletions and duplications in the Cl inhibitor gene of hereditary angioedema patients. Hum Mutat 2001 17(1) 61 70. 

Optical sensor/optrode Absorbance fluorescence Multiplex advantage, inexpensive one-way sensors Bleaching... 

Bacher JW, Elanagan LA, Smalley RL, et al. Development of a fluorescent multiplex assay for detection of MSI-High tumors. Dis Markers. 2004 20 237-250. 

Zhu, L., Stryjewski, W, Lassiter, S., and Soper, S.A., Fluorescence multiplexing with time-resolyed and spectral discrimination using a near-IR detector. Anal. Chem., 75, 2280, 2003. 

Sukhanova, A and Nabiev, I. (2008). Fluorescent nanocrystal-encoded microbeads for multiplexed cancer imaging and diagnosis. Crit. Rev. Oncol. Hematol DOI 10.1016/j.critrevonc.2008.05.006. 

Preparation of a series of phycobiliprotein tandem dyes allows multiplexed analysis of different targets in a sample. In addition, since RPE can be excited by the argon-ion laser at 488 nm, a fluorescein-labeled probe can be used concurrently with RPE alone and RPE-tandem conjugates to create a multiplexed system of different fluorescent probes that can be used simultaneously. Table 9.3 shows the different combinations of dyes that can be used in this type of assay with RPE and APC. 

Fig. 16.8 Multiplexing immobilization on paralell resonators, (a) SEM of parallel resonators (scale bar is 3 pm), (b) SEM of parallel parylene patterned ID photonic crystal resonators (scale bar is 20 pm), (c) Fluorescence micrograph of patterned capture prohes after parylene removal (scale bar is 20 pm)...

Keywords Amplification Fluorescent reporter Fluorophore FRET In vitro In vivo Labeling Lanthanide chelate Multiplexing Nanoparticle Quantum dot Transition metal complex... 

This comparison of the spectroscopic properties of the different types of fluorescent reporters underlines that semiconductor QDs and upconverting nanoparticles have no analogs in the field of organic dyes. Therefore, their unique features are unrivaled. The different molecular labels detailed here each display unique advantages that can compete with some of the favorable features of QDs and upconverting phosphors such as long lifetimes in the case of MLC systems and lanthanide chelates or very narrow emission bands for lanthanide chelates beneficial for spectral multiplexing. 

Spectral multiplexing or multicolor detection is typically performed at a single excitation wavelength, and relies on the discrimination between different fluorescent labels by their emission wavelength. Desirable optical properties of suitable fluoro-phores are a tunable Stokes shift and very narrow, preferably well-separated emission bands of simple shape. 

Grabolle M, Kapusta P, Nann T, Shu X, Ziegler J, Resch-Genger U (2009) Fluorescence lifetime multiplexing with nanocrystals and organic labels. Anal Chem 81 7807-7813... 

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