Immunofluorescence detection

Ness, J.M., Akhtar, R.S., Latham, C.B., and Roth, K.A. (2003) Combined tyramide signal amplification and quantum dots for sensitive and photostable immunofluorescence detection. J. Histochem. Cytochem. 51, 981-987. 

Indirect methods for immunofluorescent detection of multiple tissue antigens in their simplest form make use of primary antibodies that are raised in different species and accordingly can be visualized with differently labeled species-specific secondary antibodies (see Sect. 8.1). However, quite often the appropriate combination of primary antibodies from different host species is not available. A general problem relates to the fact that the available primary antibodies may originate only from one species either rabbit or mouse. When primary antibodies are raised in the same host species, the secondary species-specific antibodies can cross-react with each of the primary antibodies (Ino 2004). 

Levi, M., Sparvoli, E, Sgorbati, S., and Chiantante, D. (1990) Biotin-streptavidin immunofluorescent detection of DNA replication m root meristems through Brd Urd incorporation- cytological and microfluorimetnc applications Physiol Plantarum 79, 231-235... 

Selden, J. R., Dolbeare, F., Clair, J. H., Nichols, W. W., Miller, J. E., Kleemeyer, K. M., Hyland, R. J., and DeLuca, J. G. 1993. Statistical confirmation that immunofluorescent detection of DNA repair in human fibroblasts by measurement of bromodeoxyuridine incorporation is stoichiometric and sensitive. Cytometry 74 154-167. 

Mouse IgG Goat 1 100-1 400 Immunofluorescence detection Molecular Probes... 

Rat IgG Goat 1 50-1 200 Immunofluorescence detection Jackson, West Grove, PA, USA... 

Improved quality of autoantigen preparation (human recombinant antigen vs guinea pig liver enzyme) in ELISAs led to a diagnostic accuracy comparable to indirect immunofluorescent detection of endomysium antibodies [115, 117, 118, 120, 122-127]. These advances in ELISA technology have led to a progressive replacement of the more labor-intensive and -subjective immunofluorescent techniques. 

Estimation of autoantibodies in CD is based mainly on detection of IgA class immunoglobulins. However, CD is associated with selective IgA deficiency [131]. About 2% of CD patients are IgA deficient in Italy [132, 133] and Ireland [134]. In cases of IgA deficiency, the corresponding IgG antibodies should be evaluated. Indirect immunofluorescent detection of IgG anti-endomysium antibodies has drawbacks due to secondary antibodies against human IgG often nonspecifically binding to connective tissue fibers [135]. However, IgG class antibodies against tTG have shown high specificity for CD in cases of IgA deficiency [136-139],... 

Ward, B. B., and Cockroft, A. R. (1993). Immunofluorescence detection of the denitrifying strain Pseudomonas stutzeri (ATCC 14405) in seawater and intertidal sediment environments. Microb. Ecol. 25, 233-246. 

Rizzetto, M., Canese, M.G., Arico, S., Crivelli, O., Trepo, C., Bonino, F., Verme, G. Immunofluorescence detection of new antigen-antibody system (8/anti-8) associated to hepatitis B virus in liver and in serum of HBsAg carriers. Gut 1977 18 997-1003... 

Sharp BM, McAllen K, Shahabi NA (2005) Immunofluorescence detection of anti-CD3-e-induced delta opioid receptors by murine splenic T cells. In Infectious Diseases and Substance Abuse (Friedman H, ed), pp 141-147. New York Springer. 

Wiik A. Delineation of a standard procedure for indirect immunofluorescence detection of ANCA. APMIS Suppl 1989 6 12-13. ... 

Immunofluorescent detection of TS protein was done with the use of monoclonal antibodies, developed by in vivo immunization of Balb/c mice with homogeneous recombinant rat hepatoma TS protein as an antigen. The specific anti-rat TS antibodies recognized also T. spiralis TS, as indicated by cross-reactivity on Western blot. Localization of the enzyme was based on analysis of pictures collected by confocal microscopy. Two types of T spiralis muscle larvae preparations were studied muscle larvae isolated from mouse muscles by a procedure destroying nurse cells and muscle larvae remaining in nurse cells, isolated as an intact nurse cell preparation. 

Fig. 1. Double immunofluorescence detection of cytoplasmic cytokeratin 19 intermediate filaments (CK19) (FITC, green/ filamentous) and the cell-membrane associated connexin 43 (Cx43) gap junction protein (Cy3, red/dot-like) in the stratified squamous epithelium of human cervix. Combination of mouse monoclonal (CK19) and rabbit polyclonal (Cx43) antibodies and distinct fluorochrome-labeled secondary antibodis. Confocal laser scanning microscopy, a projection of five optical layers.

Sea Urchin. Immunofluorescence detection of lamins of sea urchin sperm nuclei and male pronuclei in vivo was originally reported by Schatten et al. (1985). Antibodies to lamin A/C and B were shown to label the poles of sea urchin sperm nuclei exclusively, suggesting that lamins were lost during spermatogenesis. Prior to fixation, however, these preparations were treated with 1% NP-40, a nonionic detergent that, tike 1% Triton X-100, has been subsequently shown to remove lateral nuclear membranes as well as lateral lamin components (Collas et al., 1995). 

Fig. 2. Immunofluorescent detection of autoantibodies to poly(ADP-ribose) polymerase. Acetone-fixed HEp-2 cells reacted with a serum containing antibodies to poly(ADP-ribose) polymerase followed by fluorescein-conjugated goat anti-human IgG. The diffuse nuclear staining with nucleolar accentuation is apparent.

Stingl G, Katz SI, Abelson LD, Mann DL. Immunofluorescent detection of human B cell tilloan-tigens on S-Ig-positive lymphocytes tmd epidermal Langerhans cells. J Immunol. 1978 120 661-4. 

Dupont E. P., E. Labonne, C. Vandevyver, U. Lehmann, E. Charbon, M. A. M. Gijs. Monolithic Silicon Chip for Immunofluorescence Detection on Single Magnetic Beads, Anal. Chem., 82, 49-52 (2010). 

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