Immunoassay thyroid stimulating hormone

A.M. Pelkkikangas, S. Jaakohuhta, T. Lovgren, and H. Harma, Simple, rapid, and sensitive thyroid-stimulating hormone immunoassay using europium(III) nanoparticle label. Anal. Chim. Acta 517, 169-176 (2004). 

Time-resolved approaches for multi-analyte immunoassays have been described recently. Simultaneous determination of LH, follicle stimulating hormone (FSH), hCG, and prolactin (PRL) in a multisite manual strip format has been reported. 88 Four microtiter wells are attached to a plastic strip, two-by-two and back-to-back, such that the wells can be read on a microtiter plate reader. In a quadruple-label format, the simultaneous quantitative determination of four analytes in dried blood spots can be done using europium, samarium, dysprosium, and terbium. 89 In this approach, thyroid-stimulating hormone, 17-a-hydroxyprogesterone, immunoreactive trypsin, and creatine kinase MM (CK-MM) isoenzyme are determined from dried blood samples spotted on filter paper in a microtiter well coated with a mixture of antibodies. Dissociative fluorescence enhancement of the four ions using cofluorescence-based enhancement solutions enables the time-resolved fluorescence of each ion to be measured through four narrow-band interference filters. 

Y.-Y. Xu, K. Pettersson, K. Blomberg, I. Hemmila, H. Mikola, and T. Lovgren, Simultaneous quadruple-label fluorometric immunoassay of thyroid-stimulating hormone, 17 othydroxypro-gesterone, immunoreactive trypsin, and creatine kinase, Clin. Chem. 38, 2038-2043 (1992). 

Fig. 7. A typical standard curve for enzyme immunoassay of thyroid stimulating hormone...

Imagawa, M., Ishikawa, E., Yoshitake, S., Tanaka, K., Kan, H., Inada, M., Imura, H., Kurosald, H., Tachibana, S., Takagi, M., Nishiura, M., Nakazawa, N., Ogawa, H., Tsunetoshi, Y., and Nakajiraa, K., A sensitive and specific sandwich enzyme immunoassay for human thyroid-stimulating hormone. Clin. Chim. Acta 126, 227-236 (1982). 

K2. Kato, N, Naruse, H., Irie, M., and Tsuji, A., Fluorophotometric enzyme immunoassay of thyroid-stimulating hormone. Anal. Biochem. 96, 419-425 (1979). 

Fig. 12.35. Quadruple-label immunoassays of four analytes (thyroid stimulating hormone, 17a-hydroxyprogesterone, immunoreactive trypsin and creatine kinase) in a single assay cuvette with...

STN24 Welch, C. and Nadkami, S. (1991). Evaluation of the Stratus II immunoassay system for thyroxine, thyroid stimulating hormone, and human chorionic gonadotropin. Clin. Chem. 37, 1041-1042, Abstr. 627. 

UUman EF, Kirakossian H, Singh S, Irvin BR, Irvine JD, Wagner DB. Luminescent oxygen channeling immunoassay (LOCI) for human thyroid stimulating hormone. In Campbell AK, Kricka LJ, Stanley PE eds, Bioiuminescence and chemiluminescence. Fundamentals and applied aspects. Chichester Wiley, 1994 6-19. 

Ismail AAA, Walker PL, Barth JH, Lewandowski KC, lones R, Burr WA. Wrong biochemistry results two case reports and observational study in 5310 patients on potentially misleading thyroid-stimulating hormone and gonadotropin immunoassay results. Clin Chem 2002 48 2023-9. 

Keller C, Novotny M, Ramp S, Brashear J, Brandt D, Abbott K, A rapid, sensitive assay for human thyroid-stimulating hormone (hTSH) on the Abbott IMx automated immunoassay system. CHn Chem 1988 34 1208. 

Kwiatkowski M, Long L, Seery J, Blake D, Travis K. An automated chemiluminescence immunoassay test for thyroid stimulating hormone (TSH). Chn Chem 1990 36 1078. 

We worked out the effective method for obelin conjugation with the other molecules - avidin, antibodies and so on. To analyze the thyroid gland hormones in sera, we synthesized the obelin conjugates with antibodies to the thyroid-stimulating hormone (TSH), antibodies to thyroxin (T4), and T4 itself. The bioluminescent labels obtained were applied for different format solid-phase immunoassay. Fig. 2 illustrates the TSH sandwich-type immunoassay, and the calibration curve built as a dependence of bioluminescent response on hormone concentration in standard sera. This curve was used to determine TSH in 34 patients sera. The results obtained closely corresponded to those provided by the RIA data (r = 0.99). 

Using the substrate of 1,2-dioxetane derivative in combination with an enhancer, we applied a chemiluminescence enzyme immunoassay (CLEIA) for TSH (Thyroid Stimulating Hormone). 

In conclusion, our substrate comprised of a new 1,2-dioxetane derivative and an enhancer was found to have excellent characteristics with a high thermal stability and a very high light yield which can be well applicable in clinical usage. CLEIA using a new chemiluminescence substrate showed highly sensitive immunoassay with TSH (Thyroid Stimulating Hormone) even less than 0.001 /tIU/mL. 

H4. Hall, R., The immunoassay of thyroid-stimulating hormone and its clinical applications. Clin. Endocrinol. 1, 115-125 (1972). 

Zhang, B., Tang, D., Liu, B., Cui, Y., Chen, H., Chen, G., 2012. Nanogold-functionalized magnetic beads with redox activity for sensitive electrochemical immunoassay of thyroid-stimulating hormone. Anal. Chim. Acta 711, 17—23. 

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