Hybridization histochemistry

Saura, J, Bleuel, Z, Ulrich, J, Mendelowitsch, A, Chen, K, Shih, JC, Malherbe, P, Da Prada, M and Richards, JG (1996) Molecular neuroanatomy of human monoamine oxidases A and B revealed by quantitative enz5mie radioautoradiography and in situ hybridization histochemistry. Neurosci. 70 755-774. 

Sato, K., Zhang, J. H., Saika, T. et al. (1991). Localization of glycine receptor alpha 1 subunit mRNA-containing neurons in the rat brain an analysis using in situ hybridization histochemistry. Neuroscience 43, 381-95. 

Mengod, G., Nguyen, H., Le, H. et al. (1990). The distribution and cellular localization of the serotonin 1C receptor mRNA in the rodent brain examined by in situ hybridization histochemistry. Comparison with receptor binding distribution. Neuroscience 35, 577-91. 

Landwehrmeyer B., Mengod G., Palacios J. Differential visualization of dopamine D2 and D3 receptor sites in the rat brain. A comparative study using in situ hybridization histochemistry and ligand binding autoradiography. Eur. J. Neurosci. 5 145, 1993. 

DePaoli A, Hurley K, Yasuda K, Reisine T, Bell GI. Distribution of kappa opioid receptor mRNA in adult mouse brain An in situ hybridization histochemistry study. Mol Cell Neurobiol 1994 5 327-335. 

Jackson, P., Dockey, D.A., Lewis, F.A., and Wells, M. (1990) Application of 1-nm gold probes on paraffin wax sections for in situ hybridization histochemistry./. Clin. Pathol. 43, 810-812. 

Kiyama, H., Emson, P.C., and Tokyama, M. (1992) In situ hybridization histochemistry using alkaline phosphatase-labeled oligodeoxynucleotide probe. In Methods in Molecular Biology, Protocols in Molecular Neurobiology, (A. Longstaff, and R Revest, eds.), Vol. 13, pp. 167-179. Humana Press, Totowa, New Jersey. 

Baldino F, Chesselet MF, Lewis ME. 1989. High resolution in situ hybridization histochemistry. Methods Enzymol 168 761-778. 

Mengod G, Vilaro MT. 1993. Analysis of receptor expression by in situ hybridization histochemistry. Receptor autoradiography principles and practice. Wharton J, Polak JM, editors. Oxford, England Oxford University Press pp. 159-170. 

Ambesi-Impiombato A, D Urso G, Muscettola G, de Bartolomeis A. 2003. Method for quantitative in situ hybridization histochemistry and image analysis applied for Homer la gene expression in rat brain. Brain Res Prot 11 189-196. 

Grino M, Zamora AJ. 1998. An in situ hybridization histochemistry technique allowing simultaneous visualization by the use of confocal microscopy of three cellular mRNA species in individual neurons. J Histochem Cytochem 46 753-759. 

Kerner JA, Standaert DA, Penney JB Jr, Young AB, Landwehr-meyer GB. 1998. Simultaneous isotopic and nonisotopic in situ hybridization histochemistry with cRNA probes. Brain Res Prot 3 22-32. 

Stern CA. 1995. Detection of multiple gene products simultaneously by in situ hybridization histochemistry and immunohistochemistry in while mounts of avian embryos. Curr Top Dev Biol 36 223-243. 

Miquel MC, Doucet E, Boni C (1991) Central serotonin 1A receptor respective distributions of encoding mRNA, receptor protein and binding sites by in situ hybridization histochemistry, radioimmunohistochemistry and autoradiographic mapping in the rat brain. Neurochem Int 19 453-465... 

Penschow, J.D., Gasser, R.B., Darling, P.E. and Mitchell, G.F. (1 993) Female-specific genomic PCR sequences from Schistosoma mansoni as probes for hybridization histochemistry. International Journal for Parasitology 23, 407-409. 

Ruda, M. A., Iadarola, M. J., Cohen, L. V., and Young, W. S. Ill (1988). In situ hybridization histochemistry and immunocytochemistry reveal an increase in spinal dynorphin biosynthesis in a rat model of peripheral inflammation and hyperalgesia. Proc. Natl. Acad. Sci. USA 85, 622—626. 

Vilaro MT, Cortes R, Gerald C, Branchek TA, Palacios JM, Mengod G. Localization of 5-HT4 receptor mRNA in rat brain by in situ hybridization histochemistry. Brain Res Mol Brain Res 1996 43 356-360. 

By in situ hybridization histochemistry, 5-HT5B receptors have been shown to be predominantly expressed in the habenular nuclei, hippocampal CA1 pyramidal cells and inferior olivary nucleus of the rat, and at weaker levels in the entorhinal cortex and NRD (210). The 5-HT5B receptor mRNA has recently been shown to be coexpressed with the plasma membrane 5-HT transporter in the NRD (217), strongly suggesting an autoreceptor as well as heteroreceptor role for 5-HT5B as well as 5-HT5A receptors. 

The 5-HT6 receptors are positively coupled to adenylate cyclase. In the absence of selective agonists and antagonists as well as radioligands, they have been initially localized in the rat brain by Northern blot analyses (218,219), in situ hybridization histochemistry (219-221), and quantitative reverse transcription followed by polymerase chain reaction (222) (see also ref. 210). These studies have established that the receptor mRNA is abundant in extrapyramidal areas such as the striatum, as well as in limbic areas such as the nucleus accumbens, olfactory tubercle, hippocampus, and hypothalamus. 

Mengod G, Pompeiano M, Martinez-Mir MI, Palacios JM. Localization of the mRNA for the 5-HT2 receptor by in situ hybridization histochemistry. Correlation with the distribution of receptor sites. Brain Res 1990 524 139-143. 

Ward RP, Hamblin MW, Lachowicz JE, Hoffman BJ, Sibley DR, Dorsa DM. Localization of serotonin subtype 6 receptor messenger mRNA in the rat brain by in situ hybridization histochemistry. Neuroscience 1995 64 1105-1011. 

Key Words Serotonin 5-HT receptor subtypes receptor autoradiography radioligands mRNA in situ hybridization histochemistry immuno-histochemistry. 

The distribution of the hybridization signal of 5-HT1A mRNA-containing cells obtained by in situ hybridization histochemistry was studied in the rat (9,10) (see also Fig. 1), in monkey (14), and human brain (29-31) (see also Fig. 2). High levels of 5-HT1A mRNA are seen in areas such as the dorsal raphe... 

The distribution of r5-HT1B and r5-HT1D receptor mRNA-containing cells has been studied by in situ hybridization histochemistry in the mouse (74), rat (75) (see also Fig. 3), guinea pig (76), monkey (44), and human (30,44,77) brain. 

The first distribution studies of the 5-HT1F receptors involved the localization of its mRNA by in situ hybridization histochemistry in the mouse (82), rat (83), and guinea pig (14,75) brains. 5-HT1F receptor mRNA is found in layer V of cortex, claustrum, striatum, several thalamic nuclei, hippocampus, some nuclei of the amygdala, and in the dorsal raphe. There are no reports on the localization of 5-HT1F receptor mRNA in human brain by in situ hybridization histochemistry. 

The anatomical visualization of 5-ht1E receptor mRNA has been done in monkey and human brain by in situ hybridization histochemistry (75) see also Fig. 4) and in the guinea pig brain (Fig. 4). The strongest signals are seen in the caudate and putamen, islands of Calleja, external and internal cortical layers (layers II and V in the monkey brain), CA fields and dentate gyms of the hippocampal formation, dorsal and ventral parts of the lateral septal nuclei, and some hypothalamic nuclei. 

The mRNA coding for 5-HT7 receptors, visualized by in situ hybridization histochemistry in the rat and guinea pig brain (229,232) see also Fig. 11), is present at high levels in discrete nuclei of the thalamus and hippocampus. Moderate levels are found in the cortex, septum, hypothalamus, centromedial amygdala, and periaqueductal gray. 

Bonaventure P, Langlois X, Leysen JE. Co-localization of 5-HT1B- and 5-HT1D receptor mRNA in serotonergic cell bodies in guinea pig dorsal raphe nucleus a double labeling in situ hybridization histochemistry study. Neurosci Lett 1998 254 113-116. 

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