Human saphenous vein endothelial cells

Figure 26. Reconstruction of the tunica intima on the inner surface of a clinically used polyethylene terephtalate vascular prosthesis. A non-modified inner surface of the prosthesis, B immobilization of defined assemblies of protein molecules (e.g., collagenfiarninin or collagen+fibrin) on the inner surface of the graft, C immunofluorescence of von Willebrand factor, a marker of the identity a differentiation of vascular endothelial cells, in human saphenous vein endothelial cells in cultures on the inner surface of a prosthesis coated with collagen and larninin, D detail of a layer of endothelial cells growing on a layer of collagen and fibrin. Note well developed talin-containing focal adhesion plaques. A, B conventional optical microscope, C, D confocal microscope Leica DM 2500 [30,31].

Urquhart, P., Parkin, S.M., Rogers, J.S., Bosley, J.A., Nicolaou, A. 2002. The effect of conjugated linoleic acid on arachidonic acid metabolism and eicosanoid production in human saphenous vein endothelial cells. Biochim. Biophys. Acta 1580, 150-160. 

Using reverse transcriptase-polymerase chain reaction, the gene product encoding for CB, receptors has been located in renal endothelial cells, mesenteric resistance arterioles, and cerebral micro vessels, which is consistent with the expression of CB, receptors in the vasculature (Deutsch et al., 1997 Darker et al., 1998 Randall et al., 1999). Others have also identified mRNA in human endothelial cells (Sugiura et al., 1998 Liu et al., 2000) and Liu et al. (2000) have identified CB, receptor binding sites by radioligand studies. Similarly, immunoreactivity to the CB, receptor has been identified on human saphenous vein endothelial cells (Bilfinger et al., 1998). 

The trans isomers of octadecenoic acid such as elaidic acid are known inhibitors of A6 desaturase. Elaidic acid is usually formed during the hardening process of vegetable oils. Unfortunately, this acid is considered to be a hypercho-lesterolemic fatty acid (14). Conjugated linoleic acid (CLA), found in dairy products, is a trans-cis isomer of octadeca-dienoic acid. CLA has been shown to downregulate the metabolism of LA (15,16). CLA also reduces production of eicosanoids in several tissues (Fig. 3). The 9c,lU-isomer of conjugated linoleic acid appears to inhibit, whereas the 10Ll2c-isomer stimulates eicosanoid production in human saphenous vein endothelial cells (16). 

Expression (Human) Tissues Leukocytes, thymus, spleen, liver, ovary Cells PBLs, neutrophils,T-cells, dendritic cells, mast cells, eosinophils, macrophages, leukocytes Tissues spleen, small intestine, placenta, lung smooth muscle, Cells bronchial smooth muscle, CD34+ hemapoietic progenitor cells, monocytes, macrophages, mast cells, eosinophils, neutrophils, PBLs, human umbilical vein endothelial cells Tissues, heart, skeletal muscle, spleen, brain, lymp node, adrenal medulla, lung, human pumonary/ saphenous vein Cells monocytes, macrophages, mast cells, eosinophils, cardiac muscle, coronary artery, PBLs... 

Quercetin and rutin suppressed photosensitized hemolysis of human erythrocytes with ho values equal to 40 p.mol l-1 and 150 jjlmt>I I 1, respectively [139]. Suppression of photohemolysis was accompanied by inhibition of lipid peroxidation. Morin inhibited oxygen radical-mediated damage induced by superoxide or peroxyl radicals to the human cells in the cardiovascular system, erythrocytes, ventricular myocytes, and saphenous vein endothelial cells [140]. Rutin protected against hemoglobin oxidation inside erythrocytes stimulated by prooxidant primaquine [141],... 

RA was also shown to inhibit the production of series-2 prostaglandins and thromboxanes in endothelial cells from human saphenous vein, which is the vein commonly used in coronary bypass surgery (Urquhart et al., 2002). 

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