Human placental endothelial cells

Antohe F, Radulescu L, Gafencu A, Ghetie V, Simionescu M. Expression of functionally active FcRn and the differentiated bidirectional transport of IgG in human placental endothelial cells. Hum Immunol 2001 62(2) 93-105. 

Placental venous endothelial cells have different characteristics depending on which part of the vasculature they have been isolated from, Tsolation, purification, and culture of primary human placental endothelial cells (HPECs) from microvessels on the venous side were reported by Jinga and coworkers (2(KX)). To gain pure cultures of HPECs, trypsin perfusion of the placental cotyledon was followed by Percoll gradient and sequential trypsinization of cultures, The,se cultures represent pure HPEC form microvessels by expressing typical patterns of markers ACE, von Willcbrand factor (typi-... 

Schutz, M., Teifel, M., and FriedI, P. (1997). Establishment of a human placental endothelial cell line with extended life span after transfection with SV 40 T-antigens. Eur. J. Cell Biol. 74, 315-120. 

Schematic representation of the mesenchymal and endothelial cascade of differentiation in a murine model (mice) and in humans (hum). All mesenchymal and endothelial cells are negative for the antigen CD45. Based on this, the dynamics of surface antigen expression along development of different mature cells derived from the mesenchymal and endothelial systems can be observed. Rounded arrows indicate selfrenewal potential. Smooth, thinner arrows indicate directions of cellular differentiation, and dotted arrows indicate possible hierarchies, but yet to be proved experimentally. The question marks indicate lack of data on the pathway or on cellular identity. The identification of CDs (clusters of differentiation) and other antigen cell markers can be found in the list of abbreviations LT-HSC, long-term hematopoietic stem cell EC, endothelial cell PDMPC, placental-derived mesenchymal progenitor cell MSC, mesenchymal stem cell MAPC, mesenchymal adult progenitor cell.

Twenty years ago, a high-affinity-binding site for uPA was demonstrated on the surface of peripheral blood monocytes and cultured cells of the human histiocytic lymphoma cell line, U937 [46]. The expression of uPAR on the cell surface of many cell types has since then been demonstrated, including a variety of neoplastic cell lines as well as nonneoplastic cells such as neutrophils, macrophages, keratinocytes, placental trophoblasts, endothelial, and smooth muscle cells [7, 33, 47-51]. The human uPAR gene has been mapped to chromosome 19ql.3 [52]. 

Explant cultures have also been used to isolate specific cell types from the placenta, Leik ei ai (2004) grew arterial smooth muscle cells from cultured small pieces of placental arteries from chorionic plate. Within 1 week, cells with uniform morphology expressing proteins similar to human aonic smooth muscle cells but clearly different from fibroblasts or endothelial cells grew- out of the expiants. These cells can be used as a general model for human arterial smooth muscle cells (I eik et ai, 2004),... 

The placental interface between maternal and fetal blood is in the chorionic villus and consists of capillary endothelial cells and cytotrophoblasts. Human and rodent placentas are hemochorial, where fetal chorionic villi bathe in lacunae of maternal blood. However, they differ in that rat chorionic villi contain a second layer of cytotrophoblasts. Sampling of human placenta should be from multiple sites because of variation in maturity in different areas and foci of degeneration. 

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