Histidine Hydrogen Exchange for Analysis of Protein Folding, Structure, and Function

Histidine Hydrogen Exchange for Analysis of Protein Folding, Structure, and Function 

Michael C. Fitzgerald, Lorrain Jin, and Due T Tran Department of Chemistry, Duke University, Durham, NC, USA 

Hydrogen Exchange Mass Spectrometry of Proteins Fundamentals, Methods, and Applications, First Edition. Edited by David D. Weis. 

The histidine H/D exchange reaction in D O follows pseudo first-order kinetics where the pseudo first-order rate constant, k, can be described by Equation 10.1 [3,5]  

In Equation 10.1, is the second-order rate constant for the bimolecular reaction in the rate-limiting step, K is the dissociation constant of the imidazolium cation, and is the autoionization constant 

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