Histidine hydrogen exchange protein folding

Histidine Hydrogen Exchange for Analysis of Protein Folding, Structure, and Function... 

In Equations 10.10 and 10.11, is the observed histidine hydrogen exchange rate. Equation 10.10 holds for folded proteins in which the histidine hydrogen exchange reaction requires the global... 

The chemical denaturant-dependent histidine hydrogen exchange protocol was initially developed and applied to several model protein systems including RNase A, myoglobin, and bovine carbonic anhydrase (BCA) II [32]. RNase A and myoglobin are known to have two-state folding behavior. BCA II is a non-two-state folding protein that is known to have a partially folded... 

In Equation 10.8, AG is the folding free energy of the protein, is the first-order rate constant of the slow hydrogen exchange reaction at the C-2 position in the imidazole side chain of an unprotected histidine, m is 54G, / 5[denaturant], T is the temperature in Kelvin, R is the ideal gas constant, and [P] is the protein concentration expressed in n-ma- equivalents. Equation 10.8 can be daived from Equation 10.9, which is commonly used in the linear extrapolation method (LEM) to analyze denaturant-induced equilibrium unfolding curves [34] ... 

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