Hydrogen exchange histidine

Histidine Hydrogen Exchange for Analysis of Protein Folding, Structure, and Function... 

Figure 10.1 Schematic representation of the mechanism of histidine hydrogen exchange. The imidazole side chain of histidine is shown with the C-2 proton/deuteron highlighted in the dotted circle...

Tran and coworkers showed that Equation 10.8, originally reported for the analysis of SUPREX data [33], could be used to determine AG values from values generated in chemical denaturant-dependent histidine hydrogen exchange experiments [32] ... 

In Equations 10.10 and 10.11, is the observed histidine hydrogen exchange rate. Equation 10.10 holds for folded proteins in which the histidine hydrogen exchange reaction requires the global... 

The chemical denaturant-dependent histidine hydrogen exchange protocol was initially developed and applied to several model protein systems including RNase A, myoglobin, and bovine carbonic anhydrase (BCA) II [32]. RNase A and myoglobin are known to have two-state folding behavior. BCA II is a non-two-state folding protein that is known to have a partially folded... 

Figure 10.7 Chemical denaturant-dependent histidine hydrogen exchange data obtained for BCA II. Data obtained on a peptide containing histidine residues 118 and 121 are shown in (a), and data obtained for a peptide containing histidine residues 93, 95, and 96, are shown in (b). The dotted arrows indicate values associated with each transition. Reproduced with permission from Ref [32]. 2012,...

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