High pressure liquid chromatography compared

Biochemical analyses of 6-OHDA-injected animals revealed a 93 percent depletion of dopamine. The tissue was assayed using electrochemical detection following separation by high-pressure liquid chromatography (Felice et al. 1978). recorded as ng/mg protein in the nucleus accumbens and compared to control rats with sham lesions (sham=65.5 4.4, lesion=4.9 1.5 t(39)=23.4). A lesion was defined as complete if 75 percent or more of the dopamine was determined to be depleted from the nucleus accumbens compared to mean sham group values. 

Rate constants and the products formed in the hydrolysis of Cl Reactive Red 194 (7.76) at 50 °C and pH values in the 10-12 region were determined by high-pressure liquid chromatography. In addition to the normal hydrolysis of the two reactive systems, the imino link between the triazine and benzene nuclei was also hydrolysed [67]. The heterobifunctional copper formazan dye Cl Reactive Blue 221 and two blue anthraquinone monofunctional reactive dyes of the bromamine acid type, namely the aminochlorotriazine Blue 5 and the sulphatoethylsulphone Blue 19, were compared in terms of their sensitivity to... 

In this report we compare several properties of hepatic microsomal AHH activity in control and DBA-treated little skates (including metabolic profiles obtained from c-benzo(a)pyrene as elucidated by high pressure liquid chromatography [HPLC]), we describe the partial purification of two different forms of cytochrome P-450 (cytochrome P-448 and cytochrome P-451) from hepatic microsomes of DBA-pretreated little skates and we report polycyclic hydrocarbon-like induction in large numbers of winter flounder assayed in Maine during June, July, and August, which was quite different than data obtained with sheepshead examined in Florida during the same period. 

CS048 Wheals, B. B. and R. N. Smith. Comparative cannabis analysis. A comparison of high-pressure liquid chromatography with other chromatographic techniques. J Chromatogr 1975 105 396-400. 

MTHF was initially measured by microbiological and radioisotope dilution assay [12, 13], and later by high-pressure liquid chromatography (HPLC) using electrochemical (EC), ultraviolet, or fluorescence detection [14-16]. Compared to other methods, EC detection is more sensitive. 

The system was applied to the determination of cephalosporin c in a broth of Cephalospolium acremonium, and was compared with a method based on high-pressure liquid chromatography (h.p.l.c.). 

Apart from the above reasons derivatisation is also required in the analyses of many of the newer pesticides which have one or more functional groups which need protection in order to facilitate GC work. Also the increased use of high pressure liquid chromatography (HPLC) necessitates pre- or post-column derivatisation since the choice of HPLC detectors is limited compared with GC. 

High-pressure liquid chromatography (HPLC) has grown into a powerful separation technique routinely applied for the separation of a variety of compounds in a variety of matrices [1], Despite its success, HPLC may still be regarded as a relatively inefficient technique, certainly when compared to, e.g., gas chromatography (GC) or capillary electrophoresis (CE). 

High sensitivity. Detecdon limits are in the range of 0.05-5 pmol/mol of unmodified parent base. This compares well to the sensidvides of commonly used methods, such as high pressure liquid chromatography with fluorescence detection (6,7), radiochromatographic analysis (8), radioimmunoassays, and enzyme-linked immunosorbent assays (9,10). 

S168 Drost, R.H., Schultz, H.U., Maes, R.A.A. and van Rossum, J.M. (1987). A comparative study of a monoclonal immunoassay reagent strip test and high pressure liquid chromatography for theophylline. Clin. Toxicol. 25, 231-241. 

Other molecular properties have been also proposed to model the hydrophobic interactions. The parachor, which is related to the surface tension of a compound (139, 140) represents mainly the intermolecular interactions in a liquid. The Hildebrand-Scott solubility parameter, 6, (141) is related to intermolecular van der Waals forces and the closely related molar attraction constant, F, is obtained by multiplying 6 by the molar volume (142). The partition coefficient between two solvents can be obtained from the solubility parameters and the molar volumes of the solute and the solvents (193). This relationship is based on regular solution theory (194) and the assumption that the partial molar volumes of the solute is not different from its molar volume. Recently this has been criticized and a new derivation was proposed (195) in which the partial molar volumes are taken into account. The molar refractivity, MR, is related to dispersion forces and can be obtained as a sum of the partial molar refractivi-ties assigned to atoms and bonds (140, 143). These parameters have been compared (144) to establish their relative applicability to correlations with biological activity. The conclusion was that logP and molecular refractivity were the best parameters. Parameters obtained from high pressure liquid chromatography (144,... 

JF Sayegh. A simplified radioimmunoassay of plasma nortriptyline in depressed patients compared with high pressure liquid chromatography and gas liquid chromatography. Neurochem Res 11 193, 1986. 

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