Cephalospolium acremonium

The system was applied to the determination of cephalosporin c in a broth of Cephalospolium acremonium, and was compared with a method based on high-pressure liquid chromatography (h.p.l.c.). 

Reusability of the Electrode. Reusability of the microbial electrode was examined with the sample solution containing 125 r /mi of phenyl acetyl-7ADCA. The results obtained are shown in Figure 6. The cephalosporin determination was performed several times a day. However, no decrease of the potential difference was observed for a week. This system was applied to the determination of cephalosporins in a fermentation broth. Cephalosporin C in the cephalospolium acremonium broth was determined by the conventional method using high pressure liquid chromatography (HPLC) (13) and the electrochemical method. The results obtained are shown in Table 1. The relative error of the determination by the microbial electrode was within 10 %. Therefore, this system can be used for the continuous determination of cephalosporin in a fermentation broth. 

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