High-performance liquid spectrometry HPLC-APCI

A variety of MS formats are widely accepted and applied in the pharmaceutical industry. The specific MS application is often defined by the sample introduction technique. The pharmaceutical applications highlighted in this article feature two types of sample introduction techniques dynamic and static. Dynamic sample introduction involves the use of high-performance liquid chromatography (HPLC) on-line with MS. The resulting liquid chromatography/mass spectrometry (LC/MS) format provides unique and enabling capabilities for pharmaceutical analysis. The electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) modes are the most widely used. Static sample introduction techniques primarily use matrix-assisted laser desorption/ionization (MALDI). ... [Pg.3419]

Stanaszek R, Piekoszewski W. 2004. Simultaneous determination of eight underivatized amphetamines in hair by high-performance liquid chromatography-atmospheric pressure chemical ionization mass spectrometry (HPLC-APCI-MS). J Anal Toxicol 28 77. [Pg.175]

Notes LOD, limit of detection MeOH, methanol EtOH, ethanol ACN, acetonitrile EtAC, ethyl acetate SPE, solid phase extraction HLB (hydrophilic lipophilic balanced) TFA, trifluoroacetic acid GC, gas chromatography TMS, trimethylsilyl MS, mass spectrometry HPLC, high-performance liquid chromatography DAD, diode array detector NMR, nuclear magnetic resonance ESI, electrospray ionization APCI, atmospheric pressure chemical ionization CE, capillary electrophoresis ECD, electrochemical detector CD, conductivity detector TLC, thin layer chromatography PDA, photodiode array detector. [Pg.65]

Strege summarized the technique of high-performance liquid chromatography-electrospray ionization mass spectrometry (HPLC-ESI-MS) in dereplication of natural products. In contrast to earlier electron impact ionization (El), ESI technique is applicable to virtually any ion in solution with a soft ionization process. A comparison of ESI with fast atom bombardment (FAB), matrix assisted laser desorption ionization (MALDI), atmospheric pressure chemical ionization (APCI) and other techniques demonstrates its superior sensitivity, compatibility and reliability when coupled with HPLC [51]. [Pg.659]

APCI = negative ion atmospheric pressure chemical ionization ECD = electron capture detector GC = gas chromatography HPLC = high performance liquid chromatography HRGC = high resolution gas chromatography MS = mass spectrometry... [Pg.139]

Most of the applications of APCI to the area of food science are in conjunction with LC-MS and high-performance liquid chromatography-mass spectrometry (HPLC-MS) and its variations. A few samples of investigations using these techniques are also included to get the flavor of what has been achieved. [Pg.268]

Jeannot, R. and Sauvard, E., High-performance liquid chromatography coupled with mass spectrometry applied to analyses of pesticides in water. Results obtained in HPLC/MS/APCI in positive mode. Analysis, TJ, 271,1999. [Pg.522]