High-performance liquid chromatography with nuclear magnetic resonance-mass

RS Plumb, J Ayrton, GJ Dear, BC Sweatman, IM Ismail. The use of preparative high performance liquid chromatography with tandem mass spectrometric directed fraction collection for the isolation and characterisation of drug metabolites in urine by nuclear magnetic resonance spectroscopy and liquid chromatography/sequential mass spectrometry. Rapid Commun Mass Spectrom 13 (10) 845-854,1999. [Pg.413]

Plumb, R.S. Ayrton, J. Dear, G.J. Sweatman, B.C. Ismail, I.M. The Use of Preparative High Performance Liquid Chromatography with Tandem Mass Spectrometric Directed Fraction Collection for the Isolation and Characterisation of Drug Metabolites in Urine by Nuclear Magnetic Resonance Spectroscopy and Liquid Chromatography/Sequential Mass Spectrometry, Rapid Commun. Mass Spectrom. 13, 845-854 (1999). [Pg.284]

Initially, simple methods such as ultraviolet-visible (UV-Vis), fluorescence or infrared (IR) spectroscopy were proposed in order to estimate the total amount of antioxidants in various food samples. However, coupled methods such as gas chromatography-mass spectrometry (GC-MS), high performance liquid chromatography with ultraviolet-visible (HPLC-UV-Vis) or nuclear magnetic resonance detector (HPLC-NMR) and liquid chromatography-mass spectrometry (LC-MS) are employed more to quantify individual tocols or carotens from various corn-based food samples. In this chapter all these methods of analysis will be briefly described. [Pg.30]

Taylor SD, Wright B, Clayton E, Wilson ID, Practical aspects of the use of high performance liquid chromatography combined with simultaneous Nuclear Magnetic Resonance and Mass Spectrometry, Rapid Commun. Mass Spectrom., 12 1732-1736, 1998. [Pg.310]

High-performance liquid chromatography (HPLC) has emerged as one of the most useful tools for the bioanalytical laboratory. This is a powerful stand-alone method that can be used for the purification, separation and quantification of biomolecules. Notably HPLC analysis can be combined with other high-tech methods such as mass spectrometry (MS) or nuclear magnetic resonance (NMR) spectroscopy to enable the identification of biomolecules. [Pg.154]

Ramm, M. et al. Rapid analysis of nucleotide-activated sugars by high-performance liquid chromatography coupled with diode-array detection, electrospray ionization mass spectrometry and nuclear magnetic resonance. J. Chromatogr. A. 2004, 1034,139-148. [Pg.159]

D. Bao, V.Thanabal, and W. F. Pool, Determination of tacrine metabolites in microsomal incubate by high-performance liquid chromatography-nuclear magnetic resonance/mass spectrometry with a column trapping system, /. Pharma. Biomed. Anal. 28 (2002), 23-30. [Pg.935]

PBO was found to be unstable when subjected to add hydrolysis- When JUC -PBO was refluxed with 1 mol L HCl for 1 hour, a single fluorescent radioactive compound was formed. This compound was isolated by preparative high-performance liquid chromatography (IIPLC) and identified by gas chromatography-mass spectroscopy (GC-MS), high-resolution MS, nuclear magnetic resonance (NMR), and comparison of HPLC retention time with a synthetic standard. [Pg.155]

The purity of protected amino acids is especially important for the synthesis of longer peptides. Standard techniques such as melting point determination, nuclear magnetic resonance (NMR) spectroscopy, mass spectrometry, and optical rotation are effective means of characterization. The optical purity can also be evaluated by high-performance liquid chromatography (HPLC) after derivatization with Marfey s reagent [216,217]. The advanced Marfey method refers to analysis by mass spectrometry after derivatization with Marfey s reagent [218-221]. Purification of side-chain protected amino acids by recrystallization is usually sufficient. [Pg.162]