High-performance liquid chromatography with nuclear magnetic resonance

D. Bao, V.Thanabal, and W. F. Pool, Determination of tacrine metabolites in microsomal incubate by high-performance liquid chromatography-nuclear magnetic resonance/mass spectrometry with a column trapping system, /. Pharma. Biomed. Anal. 28 (2002), 23-30. [Pg.935]

The purity of protected amino acids is especially important for the synthesis of longer peptides. Standard techniques such as melting point determination, nuclear magnetic resonance (NMR) spectroscopy, mass spectrometry, and optical rotation are effective means of characterization. The optical purity can also be evaluated by high-performance liquid chromatography (HPLC) after derivatization with Marfey s reagent [216,217]. The advanced Marfey method refers to analysis by mass spectrometry after derivatization with Marfey s reagent [218-221]. Purification of side-chain protected amino acids by recrystallization is usually sufficient. [Pg.162]

Strohschein, S., Pursch, M., and Albert, K., Hyphenation of high performance liquid chromatography with nuclear magnetic resonance spectroscopy for the characterization of (3-carotene isomers employing a Cjq stationary phase, J. Pharm. Biom. Anal., 21, 669, 1999. [Pg.476]

Because carotenoids are light- and oxygen-sensitive, a closed-loop hyphenated technique such as the on-line coupling of high performance liquid chromatography (HPLC) together with nuclear magnetic resonance (NMR) spectroscopy can be used for the artifact-free structural determination of the different isomers. [Pg.61]

Both vapor-phase chromatography and high performance liquid chromatography, along with nuclear magnetic resonance spectroscopy, have been used for isomer and composition analysis. [Pg.457]

Korhammer SA, Bernruether A, Hyphenation of high-performance liquid chromatography (HPLC) and other chromatographic techniques (SFC, GPC, GC, CE) with nuclear magnetic resonance (NMR) A review, J. Anal. Chem., 354 131-135, 1996. [Pg.308]

Taylor SD, Wright B, Clayton E, Wilson ID, Practical aspects of the use of high performance liquid chromatography combined with simultaneous Nuclear Magnetic Resonance and Mass Spectrometry, Rapid Commun. Mass Spectrom., 12 1732-1736, 1998. [Pg.310]

High-performance liquid chromatography (HPLC) has emerged as one of the most useful tools for the bioanalytical laboratory. This is a powerful stand-alone method that can be used for the purification, separation and quantification of biomolecules. Notably HPLC analysis can be combined with other high-tech methods such as mass spectrometry (MS) or nuclear magnetic resonance (NMR) spectroscopy to enable the identification of biomolecules. [Pg.154]

Ramm, M. et al. Rapid analysis of nucleotide-activated sugars by high-performance liquid chromatography coupled with diode-array detection, electrospray ionization mass spectrometry and nuclear magnetic resonance. J. Chromatogr. A. 2004, 1034,139-148. [Pg.159]