High-performance liquid chromatography lipid analysis

Passi, S., Rothschildboros, M.C., Fasella, P., Nazzaroporro, M. and Whitehouse, D. (1981) An application of high performance liquid chromatography to analysis of lipids in archaeological samples. Journal of Lipid Research 22, 778 784. 

The purity of all lipids and anthracyclines exceeded 98% based on thin-layer chromatography (TLC) and/or high-performance liquid chromatography (HPLC) analysis, performed as described by Barenholz and coworkers (38,49,50). 

MA Rouet-Mayer, O Valentova, E Simond-Cote, J Daussant, C Thevenot. Critical analysis of phospholipid hydrolyzing activities in ripening tomato fruits. Study by spectrofluorimetry and high performance liquid chromatography. Lipids 30 739-746, 1995. 

Christie, W. W. (1997). Analysis of fatty acids by high-performance liquid chromatography. Lipid Technol. 9,124—126. 

There are two main procedures for measuring PI 3-kinase activity which measure lipid kinase activity in intact cells or broken cell lysates respectively, and both rely on detecting the transfer of the y- phosphate of ATP to the D-3 position of the inositol head group of phosphoinositide lipids. The first method relies on metabolic labeling of intact cellular pools of ATP with [32P]Pi followed by lipid extraction (3,4) and separation of the phosphorylated lipids by high-performance liquid chromatography (HPLC) analysis (5). The advantages of this procedure are ... 

Takagi, T., and Itabashi, Y. (1981) Occurrence of Mixtures of Geometrical Isomers of Conjugated Octadecatrienoic Acids in Some Seed Oils Analysis by Open-Tubular Gas Liquid Chromatography and High Performance Liquid Chromatography, Lipids 16,546-551. 

Chan, H.W.-S. and Levett, G. Autoxidation of methyl linolenate analysis of methyl hydroxyhnolenate isomers by high performance liquid chromatography. Lipids 12,837-840 (1977b). 

Takagi, T. Ando, Y. Stereospecific analysis of triscy- n-glycerols by chiral high-performance liquid chromatography. Lipids 1991, 26, 542-547. 

High performance liquid chromatography (HPLC) has been by far the most important method for separating chlorophylls. Open column chromatography and thin layer chromatography are still used for clean-up procedures to isolate and separate carotenoids and other lipids from chlorophylls and for preparative applications, but both are losing importance for analytical purposes due to their low resolution and have been replaced by more effective techniques like solid phase, supercritical fluid extraction and counter current chromatography. The whole analysis should be as brief as possible, since each additional step is a potential source of epimers and allomers. 

Fig. 4.5.2 Actual strategies for CDG diagnosis. Initial investigations on CDG patients are routinely carried out by isoelectric focusing (IEF) of serum transferrin. With a CDG type I pattern, subsequent analysis should imply determination of phosphomannomutase (PMM) and phos-phomannose isomerase (PMI) activities. Further studies, like analysis of the lipid-linked- and protein-bound-oligosaccharides, determination of enzyme or sugar transporter activities and molecular biology studies often have to be performed in more specialised laboratories. HPLC High-performance liquid chromatography, TLC thin-layer chromatography...

Caboni, M.F. and Rodriguez-Estrada, M.T. 1997. High-performance liquid chromatography coupled to evaporative light scattering detection in lipid analysis Some application. Seminars in Food Analysis 2 159-169. 

Fig. 30 Silver ion high-performance liquid chromatography (Ag-HPLC-FID) with flame ionization detector (FID) analysis of the triacylglycerols of chromatographed Crepis alpina seed oil. Ag-HPLC-FID conditions 0.5-mg sample 5-micron Chromspher Lipids column (Chrompack International, Middelburg, The Netherlands) (4.6 X 250 mm) mobile phase 0.5% acetonitrile in hexane (v/v) flow rate 1.0 ml/min FID. Chromatogram peak triacylglycerol fatty acid abbreviations S, saturated (palmitic and stearic) O, oleic L, linoleic and Cr, crepenynoic fatty acids.

However, in a more recent paper, Caboni et al. described that the aforementioned method was not applicable for all types of food products (32). Hence, only 30% and 53% of the PL of a total lipid extract of cheese and dried egg powder, respectively, were recovered. High-performance liquid chromatography analysis revealed that the acidic phospholipids PG and PI were not... 

RA Moreau, PT Asmann, HA Norman. Analysis of major classes of plant lipids by high performance liquid chromatography with flame ionization detection. Phytochemistry 29 2461-2466, 1990. 

RA Moreau, MJ Powell, SF Osman, BD Whitaker, WF Fett, L Roth, DJ O Brien. Analysis of intact hopanoids and other lipids from the bacterium Zymomonas mobilis by high performance liquid chromatography. Anal Biochem 224 293-301, 1995. 

NU Olsson, AJ Harding, C Harper, N Salem Jr. High performance liquid chromatography method with light scattering detection for measurements of lipid class composition analysis of brains from alcoholics. J Chromatogr B 681 213—218, 1996. 

KC Amoldsson, P Kaufmann. Lipid class analysis by normal phase high performance liquid chromatography, development and optimization using multivariate methods. Chromatographia 38 317-324, 1994. 

Ullman, M.D., McCluer, R.H. Quantitative analysis of plasma neutral glycosphingolipids by high performance liquid chromatography of their perbenzoyl derivatives. J. Lipid Res., 1977, 18, 371-377. 

Iwamori, M. Costello, C. and Moser, H.W. Analysis and quantitation of free ceramide containing nonhydroxy and 2-hydroxy fatty acids, and phytosphingosine by high-performance liquid chromatography. J. Lipid Res., 1979, 20, 86. 

Christie, W.W. (1995) Silver ion high-performance liquid chromatography, in New Trends in Lipid and Lipoprotein Analysis (eds J.-L. Sebedio and E.G. Perkins), AOAC Press, Champaign, Illinois, USA, pp. 59-74. 

Also extracted from native HZ were polar hydroxylated fatty acids derived presumably from cellular arachidonic and linoleic acids. Native HZ was purified from infected RBCs by a series of centrifugation steps followed by organic extraction of its lipid coat. Analysis of the lipid coat revealed the presence of hydroxylated polyunsaturated fatty acids. These polar lipids were separated using reverse phase-, normal phase-, and chiral phase high performance liquid chromatography and subsequently examined by GC-MS. Native FIZ lipid coat GC-MS analysis revealed the presence of 15-, 12-, 11-, 9-, 8-, and 5-hydroxyeicosatetraenoic acids (HETEs) as well as 13- and 9-hydroxyoctadecadienoic acids (HODEs) (35). 

Hopmans E. C., Schouten S., Pancost R. D., van derMeerM. J. T., and Sinninghe Damste J. S. (2000) Analysis of intact tetraether lipids in archaeal cell material and sediments using high performance liquid chromatography/atmospheric pressure ionization mass spectrometry. Rapid Common. Mass. Spectrom. 14, 585-589. 

The following chapters in Advances in Lipid Methodology, Volume 3 (Cl) Chapter 3, Separation of phospholipid classes by high-performance liquid chromatography. W. W. Christie (C2) Chapter 6, Plant glycolipids Structure, isolation and analysis, E. Heinz. 

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