High-performance chromatography apparatus

SK Hjerten, F Kilar, JL Liao, A Chen, C Siebert, M Zhu. Carrier-free zone electrophoresis, displacement electrophoresis and isoelectric focusing in a high-performance electrophoresis apparatus. J Chromatography 403 47-61, 1987. 

H. Yamamoto, T. Manabe and T. Okuyama, Apparatus for coupled high-performance liquid chromatography and capillary electrophoresis in the analysis of complex protein mixtures , 7. Chromatogr. 515 659-666 (1990). 

High performance liquid chromatography (HPLC) apparatus (isocratic system) fitted with a 2-mL sample loop. 

Quinn, H. M., Menapace, R. A., and Oberhauser, C. J. (1998). High performance liquid chromatography method and apparatus. U. S. Patent 5,795,469, Cohesive Technologies, Acton, MA. 

Apparatus (See Chromatography, Appendix IIA.) Use a high-performance liquid chromatograph operated at room temperature with a 10-p.m particle size, 30-cm x 4-mm (id), C18 reverse-phase column (jxBondapak C18 column, Waters Corp., 34-T Maple Street, Milford, MA 01757, or equivalent). Maintain the Mobile Phase at a pressure and flow rate (typically 2.0 mL/min) capable of giving the required elution time (see System Suitability in High-Performance Liquid Chromatography). Use an ultraviolet detector that monitors absorption at 254 nm (0.2 to 0.1 AUFS range). 

Apparatus (See Chromatography, Appendix IIA.) Use a suitable high-performance liquid chromatography system such as described in Standard Analytical Methods of the Corn Refiners Association, equipped with a 22- to 31-cm stainless-steel column, or equivalent, a strip-chart recorder, and a differential refractometer detector maintained at 45° 0.005°. 

Note Chromatographic separations may also be characterized according to the type of instrumentations or apparatus used. The types of chromatography that may be used in the Food Chemicals Codex are column, thin-layer, gas, and high-pressure or high-performance liquid chromatography. 

Apparatus Use a suitable high-performance liquid chromatography system (see Chromatography, Appendix IIA) equipped with a dual wavelength detector system such that the effluent can be monitored serially at 254 nm and 325 to 385 nm (wide-band pass). Use a 1-m x 2.1-mm (id) column, or equivalent, packed with a strong anion-exchange resin (Dupont No. 830950405, or equivalent). 

Apparatus Use a suitable high-performance liquid chromatographic system (see Chromatography, Appendix HA) equipped with a differential refractometer detector, a precol-... 

HSCCC-thermospray (TSP) MS was initiated using an analytical HSCCC apparatus of a 5-cm revolution radius, equipped with a 0.85-mm-inner diameter (i.d.) polytetrafluoroethylene (PTFE) column at 2000 rpm [6-8]. Directly interfacing HSCCC to the MS produced, however, a problem in that the high back-pressure generated by the TSP vaporizer often damaged the HSCCC column. To overcome this problem, an additional high-performance liquid chromatography... 

Fig. 3 Chromatograms obtained by pH zone-refining CCC. (A) Separation of CBZ(Z) dipeptides. Experimental conditions are as follows apparatus type-J multilayer CPC (PC Inc., Potomac, MD, USA) with a 10-cm revolution radius column multilayer coil, 1.6-mm ID, 325 mL capacity solvent system methyl tert-h xiy ether/acetonitrile/water (2 2 3), 16 mM TEA in organic phase (pH 1.83), and 5.5 mM NH3 in aqueous phase (pH 10.62) sample eight CBZ(carbobenzyloxy) dipeptides as indicated in the chromatogram, each 100 mg in 50 mL of solvent (25 mL in each phase) flow rate 3.3 mL/hr in head-to-tail elution mode detection 206 nm revolution 800 rpm retention of stationary phase 65.1%. (B) Separation of bacitracin complex. High-performance liquid chromatography (HPLC) analysis indicated that two major components, bacitracins A and B, were isolated in peaks 3 and 5, respectively. Experimental conditions are as follows apparatus and column same as above solvent system methyl r -butyl ether/ acetonitrile/water (4 1 5), 40 mM triethylamine, 10% DEHPA in the organic stationary phase, and 20 mM HCl in aqueous mobile phase flow rate 3 mL/min sample 5 g of bacitracin dissolved in 40 mL of solvent (20 mL in each phase) revolution 800 rpm detection 280 nm.

The experiments within the frame of the REHE project were performed in the aqueous phase in a discontinuous, batch-wise manner. It was necessary, in order to get a statistically significant result, to repeat the same experiment several hundred or even several thousand times with a cycle time of typically 45 s. These studies were performed with the Automatic Rapid Chemistry Apparatus (ARCA) II (SchSdel et al, 1989), a computer-controlled apparatus for fast, repetitive high-performance liquid chromatography (HPLC) separations. A schematic of the ARCA II components is shown in Figure 6.2. 

The apparatus used for SEC is relatively simple isocratic high-performance liquid chromatography (HPLC) equipment, as shown in Figure 8. The principal components are a precision high-pressure solvent delivery system, detector, and a column with particles having appropriate pore size. Detection is conveniently accomplished with a UV photometer or spectro-... 

High-performance liquid chromatography apparatus consists of four principal parts ... 

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