High-performance anion-exchange carbohydrate analysis

Lee, YC (1990) High-performance anion-exchange chromatography for carbohydrate analysis. AnalBiochem 189 151-162... 

De Ruiter, G. A., Schols, H. A., Voragen, A. G. J., and Rombouts, F. M., Carbohydrate analysis of water-soluble uronic acid-containing polysaccharides with high-performance anion-exchange chromatography using methanolysis combined with TFA hydrolysis is superior to four other methods, Anal. Biochem., 207, 176, 1992. 

Das, H., Jayaraman, V., and Bhattacharya, J., Carbohydrate analysis of bradyrhizobial (NC 92) lipopolysaccharides by high-performance-anion exchange chromatography with pulsed amperometric detection, Biosci. Rep., 19, 219, 1999. 

T. R. I. Cataldi. C. Campa. and G. E. De Benedetto, Carbohydrate Analysis by High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection, Fresenius J. Anal. Chem. 2000, 368, 739. 

Tommaso R, Cataldi I, Campa C, and De Benedetto GE (2000) Carbohydrate analysis by high-performance anion-exchange chromatography with pulsed amperomet-ric detection the potential is still growing. Fresenius Journal of Analytical Chemistry 368 739-758. 

The concentrations of monomeric carbohydrates were determined using high performance anion-exchange chromatography (HPAEC, Dionex). The content of mannose in the filtrate and in the filtrate after acid hydrolysis (using 0.5 M sulfuric acid at 120° C for 4 hours) was determined. Using this data and data from raw material analysis, the yield of mannose residues in oligo- and polysaccharides in the filtrates were determined (2, 5). 

As discussed above, the NMR method offers many advantages compared to techniques such as HPLC and a related technique, High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection (HPAEC-PAD) which are widely applied in the analysis of lignocellulosic hydrolyzates (5). Since most carbohydrate chromatography columns separate molecules based... 

Henshall, A., High-performance anion exchange chromatography with pulsed amperometric detection (HPAE-PAD) A powerful tool for the analysis of dietary fiber and complex carbohydrates. Complex Carbohydrates in Foods, Cho, S.S., Prosky, L., and Dreher, M. (Eds.), Marcel Dekker, Inc., New York, p. 267, 1999. 

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